Native elongating transcript sequencing reveals global anti-correlation between sense and antisense nascent transcription in fission yeast

Autor: Maxime Wery, Hervé Vennin-Rendos, Daniel Gautheret, Mayuko Yoda, Marc Descrimes, Damien Hermand, Antonin Morillon, Camille Gautier, Valérie Migeot
Přispěvatelé: Dynamique de l'information génétique : bases fondamentales et cancer (DIG CANCER), Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris]-Université Pierre et Marie Curie - Paris 6 (UPMC), Institut Curie [Paris], Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris]-Sorbonne Université (SU), Université de Namur [Namur] (UNamur), Université Paris-Saclay
Rok vydání: 2017
Předmět:
0301 basic medicine
antisense transcription
Transcription
Genetic
RNA Stability
Histone Chaperones/metabolism
Peptide Chain Elongation
Translational
NET-seq
Biology
Exo2
Article
03 medical and health sciences
lncRNA
Genetic
RNA interference
Transcription (biology)
Schizosaccharomyces/genetics
Gene Expression Regulation
Fungal
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Genomics [q-bio.GN]
Schizosaccharomyces
Sense (molecular biology)
Gene expression
Histone code
Histone Chaperones
RNA
Antisense
Molecular Biology
Gene
Genetics
Regulation of gene expression
Schizosaccharomyces pombe Proteins/metabolism
Messenger RNA
Peptide Chain Elongation
Sequence Analysis
RNA
Translational
Antisense/biosynthesis
fission yeast
Histone Code
Meiosis
Fungal
030104 developmental biology
Gene Expression Regulation
Meiosis/genetics
RNA
RNA Interference
Schizosaccharomyces pombe Proteins
Sequence Analysis
Transcription
Zdroj: RNA
RNA, Cold Spring Harbor Laboratory Press, 2018, 24 (2), pp.196-208. ⟨10.1261/rna.063446.117⟩
ISSN: 1469-9001
1355-8382
DOI: 10.1261/rna.063446.117
Popis: International audience; Antisense transcription can regulate sense gene expression. However, previous annotations of antisense transcription units have been based on detection of mature antisense long noncoding (aslnc)RNAs by RNA-seq and/or microarrays, only giving a partial view of the antisense transcription landscape and incomplete molecular bases for antisense-mediated regulation. Here, we used native elongating transcript sequencing to map genome-wide nascent antisense transcription in fission yeast. Strikingly, antisense transcription was detected for most protein-coding genes, correlating with low sense transcription, especially when overlapping the mRNA start site. RNA profiling revealed that the resulting aslncRNAs mainly correspond to cryptic Xrn1/Exo2-sensitive transcripts (XUTs). ChIP-seq analyses showed that antisense (as)XUT's expression is associated with specific histone modification patterns. Finally, we showed that asXUTs are controlled by the histone chaperone Spt6 and respond to meiosis induction, in both cases anti-correlating with levels of the paired-sense mRNAs, supporting physiological significance to antisense-mediated gene attenuation. Our work highlights that antisense transcription is much more extended than anticipated and might constitute an additional nonpromoter determinant of gene regulation complexity.
Databáze: OpenAIRE
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