Ampicillin triggers the release of Pal in toxic vesicles from Escherichia coli
| Autor: | Carlie McNamara, Morgan Bauer, Leslie Gallardo, Emma Snyder, Judith Hellman, Anna Konovalova, Michael E. Pichichero, Susan (Shi) Cheng, Mark Zavorin, Lea Vacca Michel, Jeanetta Pierce, Niaya Jackson |
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| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
Lipoproteins Levofloxacin Microbial Sensitivity Tests Peptidoglycan medicine.disease_cause Microbiology chemistry.chemical_compound Western blot Sepsis Ampicillin Escherichia coli medicine Humans Pharmacology (medical) medicine.diagnostic_test Escherichia coli Proteins Vesicle Aminoglycoside General Medicine humanities Anti-Bacterial Agents Infectious Diseases chemistry Gentamicin Gentamicins Bacterial outer membrane Bacterial Outer Membrane Proteins medicine.drug |
| Zdroj: | International Journal of Antimicrobial Agents. 56:106163 |
| ISSN: | 0924-8579 |
| DOI: | 10.1016/j.ijantimicag.2020.106163 |
| Popis: | In addition to lipopolysaccharides (LPS), outer membrane proteins - Lpp, OmpA and peptidoglycan-associated lipoprotein (Pal) - are part of the outer membrane of Escherichia coli and are proposed to contribute to bacterial sepsis-related inflammation. This study showed that ampicillin (a β-lactam antibiotic) enhances Pal's release from Escherichia coli to a greater extent than gentamicin and levofloxacin (aminoglycoside and quinolone antibiotics, respectively). It is proposed that the majority of Pal is released in outer membrane vesicles (OMVs), which also contain LPS and other outer membrane and periplasmic proteins. The OMVs were purified by ultracentrifugation and characterised by transmission electron microscopy and nanoparticle tracking analysis, and Pal and other E. coli proteins were detected by Western blot. It also proposed that sepsis treatments using certain β-lactam antibiotics may further aggravate the over-exuberant inflammatory response by enhancing the release of Pal and LPS in OMVs. |
| Databáze: | OpenAIRE |
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