Steviol glycosides targeted analysis in leaves of Stevia rebaudiana (Bertoni) from plants cultivated under chilling stress conditions
| Autor: | Gilda D'Urso, Cosimo Pizza, Paola Montoro, Taoufik Bettaieb, Salah Rezgui, Sihem Soufi |
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| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
Steviol Kaurane 01 natural sciences Analytical Chemistry chemistry.chemical_compound Tandem Mass Spectrometry Botany Stevioside Food science LC-ESI-QqQ-MS/MS Chlorophyll fluorescence Chromatography High Pressure Liquid Steviol glycosides chemistry.chemical_classification Chromatography Principal Component Analysis biology Plant Extracts LC-ESI-FT (Orbitrap) MS Principal component analysis Cold Temperature Diterpenes Kaurane Food Science 010401 analytical chemistry food and beverages Glycoside General Medicine biology.organism_classification Stevia 0104 chemical sciences Stevia rebaudiana chemistry High Pressure Liquid Diterpenes Rebaudioside A Salicylic acid 010606 plant biology & botany |
| Zdroj: | Food chemistry. 190 |
| ISSN: | 1873-7072 |
| Popis: | Stevia rebaudiana is an important agricultural crop for the production of a high-potency natural sweetener, sensitive to low temperature during the developmental stage. Stimulation of chilling stress with a pre-treatment with endogenous signalling components and in particular with salicylic acid (SA), hydrogen peroxide (H2O2), 6-benzylaminopurine (BAP) and calcium chloride (CaCl2) could induce tolerance to chilling and could constitute a suitable way to maintain quality and quantity of steviol glycosides under controlled artificial environment. In the present work the effects of different putative signalling molecules on the morpho-physiological parameters were evaluated, and a specific method for the quali-quantitative analysis of steviol glycosides in S. rebaudiana plants cultivated under controlled conditions was developed, by using LC-ESI-FT (Orbitrap) MS, LC-ESI-QqQ-MS/MS and multivariate data analysis. This approach underlined that the pre-treatment has influence on the production of secondary metabolites. In particular Stevia plants characterised by higher contents of rebaudioside A and stevioside, were identified. |
| Databáze: | OpenAIRE |
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