New Overlapping Gene Encoded by the Cucumber Mosaic Virus Genome
| Autor: | Beau J. Anderson, Robert H. Symons, Shou-Wei Ding, Helen R. Haase |
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| Rok vydání: | 1994 |
| Předmět: |
Reading Frames
Genes Viral Recombinant Fusion Proteins Molecular Sequence Data RNA-dependent RNA polymerase Genome Viral Biology Cucumovirus Cucumber mosaic virus Viral Proteins Sequence Homology Nucleic Acid Virology Vegetables Gene expression Escherichia coli Amino Acid Sequence RNA Messenger Cloning Molecular Subgenomic mRNA Genetics Base Sequence Sequence Homology Amino Acid Intron RNA Non-coding RNA Biological Evolution RNA editing RNA Viral |
| Zdroj: | Virology. 198:593-601 |
| ISSN: | 0042-6822 |
| DOI: | 10.1006/viro.1994.1071 |
| Popis: | Cucumber mosaic virus (CMV) contains three genomic RNAs (RNAs 1, 2, and 3) and a subgenomic RNA (RNA 4), a shared feature of the Bromoviridae family which includes cucumoviruses, bromoviruses, alfalfa mosaic virus, and ilarviruses. We describe in this paper the molecular characterization of a novel subgenomic RNA of the Q strain of CMV (Q-CMV), RNA 4A, which was first reported in 1973 (K. W. C. Peden and R. H. Symons, Virology 53, 487-492, 1973). RNA 4A is 682 nucleotides and is identical in sequence to the 3'-terminal 682 nucleotides of RNA 2. RNA 4A encodes a small open reading frame (ORF) of 100 codons, which, in RNA 2, overlaps the C-terminal portion of the major 2a gene; thus it is likely that RNA 4A functions as the mRNA for the in vivo expression of the ORF, called ORF 2b. Polyclonal antibodies raised against a 2b fusion protein expressed in Escherichia coli specifically detected the 2b gene product in Q-CMV-infected cucumber plants by Western immunoblotting. Examination of published viral RNA sequences revealed the conservation of ORF 2b in all of the four other cucumoviruses sequenced to date; however, it is absent from the rest of the Bromoviridae. We suggest that the proposed ORF 2b may be expressed in other cucumoviruses, most likely via 4A-like subgenomic RNAs, and that the predicted gene product may have a unique functional role in the infection process of cucumoviruses. |
| Databáze: | OpenAIRE |
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