Biochemical, genetic and transcriptional characterization of multibacteriocin production by the anti-pneumococcal dairy strain Streptococcus infantarius LP90

Autor: Ingolf F. Nes, Pablo E. Hernández, Dzung B. Diep, Javier Feito, Cristina Campanero, Luis M. Cintas, Rosa del Campo, Estefanía Muñoz-Atienza, Sara Arbulu, Carmen Herranz
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0106 biological sciences
Artificial Gene Amplification and Extension
Protein Sequencing
Toxicology
Pathology and Laboratory Medicine
medicine.disease_cause
Polymerase Chain Reaction
Physical Chemistry
01 natural sciences
Bacteriocins
Antibiotics
Medicine and Health Sciences
Toxins
0303 health sciences
Multidisciplinary
Antimicrobials
Streptococcus
Structural gene
Drugs
food and beverages
Pneumococcus
Genomics
Antimicrobial
Bacterial Pathogens
Anti-Bacterial Agents
Chemistry
Molecular Mass
Pneumococcal infections
Medical Microbiology
Physical Sciences
Medicine
Pathogens
Research Article
Science
Toxic Agents
Bacterial Toxins
Antimicrobial peptides
Biology
Research and Analysis Methods
Streptococcus infantarius
Microbiology
Pneumococcal Infections
03 medical and health sciences
Bacteriocin
Microbial Control
010608 biotechnology
Genetics
medicine
Humans
Amino Acid Sequence
Molecular Biology Techniques
Gene Prediction
Sequencing Techniques
Microbial Pathogens
Molecular Biology
Bacteriocin immunity
Pharmacology
Bacteria
030306 microbiology
Organisms
Biology and Life Sciences
Computational Biology
Genome Analysis
medicine.disease
Chemical Properties
bacteria
Zdroj: PLoS ONE, Vol 15, Iss 3, p e0229417 (2020)
PLoS ONE
ISSN: 1932-6203
Popis: Streptococcus pneumoniae infections are one of the major causes of morbility and mortality worldwide. Although vaccination and antibiotherapy constitute fundamental and complementary strategies against pneumococcal infections, they present some limitations including the increase in non-vaccine serotypes and the emergence of multidrug-resistances, respectively. Ribosomally-synthesized antimicrobial peptides (i.e. bacteriocins) produced by Lactic Acid Bacteria (LAB) may represent an alternative or complementary strategy to antibiotics for the control of pneumococal infections. We tested the antimicrobial activity of 37 bacteriocinogenic LAB, isolated from food and other sources, against clinical S. pneumoniae strains. Streptococcus infantarius subsp. infantarius LP90, isolated from Venezuelan water-buffalo milk, was selected because of its broad and strong anti-pneumococcal spectrum. The in vitro safety assessment of S. infantarius LP90 revealed that it may be considered avirulent. The analysis of a 19,539-bp cluster showed the presence of 29 putative open reading frames (ORFs), including the genes encoding 8 new class II-bacteriocins, as well as the proteins involved in their secretion, immunity and regulation. Transcriptional analyses evidenced that the induction factor (IF) structural gene, the bacteriocin/IF transporter genes, the bacteriocin structural genes and most of the bacteriocin immunity genes were transcribed. MALDI-TOF analyses of peptides purified using different multichromatographic procedures revealed that the dairy strain S. infantarius LP90 produces at least 6 bacteriocins, including infantaricin A1, a novel anti-pneumococcal two-peptide bacteriocin.
Databáze: OpenAIRE
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