Activity and Stability of Native and Modified Subtilisins in Various Media
| Autor: | T. I. Velichko, A. V. Bacheva, Alexander K. Gladilin, E. N. Lysogorskaya, A. V. Belyaeva, Vladimir I. Lozinsky, O. V. Baibak, E. S. Oksenoit, I. Yu. Filippova |
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| Rok vydání: | 2003 |
| Předmět: |
endocrine system
Vinyl alcohol Acrylic Resins Peptide Biochemistry Hydrolysis chemistry.chemical_compound Bacterial Proteins Enzyme Stability Polymer chemistry Organic chemistry Subtilisins Acrylic acid chemistry.chemical_classification Aqueous solution Chemistry fungi Subtilisin General Medicine Enzymes Immobilized Kinetics enzymes and coenzymes (carbohydrates) Covalent bond Polyvinyl Alcohol biological sciences Peptides hormones hormone substitutes and hormone antagonists Bacillus subtilis |
| Zdroj: | Biochemistry (Moscow). 68:1261-1266 |
| ISSN: | 0006-2979 |
| DOI: | 10.1023/b:biry.0000009142.88974.85 |
| Popis: | The activity and stability of native subtilisin 72, its complex with poly(acrylic acid), and subtilisin covalently attached to poly(vinyl alcohol) cryogel were studied in aqueous and organic media by hydrolysis of specific chromogenic peptide substrates. Kinetic parameters of the hydrolysis of Glp-Ala-Ala-Leu-pNA by native subtilisin and its complex with poly(acrylic acid) were determined. Based on the comparative study of stability of native and modified subtilisins in media of various compositions, it was established that covalent immobilization of subtilisin on poly(vinyl alcohol) cryogel is the most effective approach to improve enzyme stability in water as well as in mixtures with low water content. |
| Databáze: | OpenAIRE |
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