Dynamics of Peripheral Blood Lymphocyte Subpopulations in the Acute and Subacute Phase of Legionnaires’ Disease
| Autor: | Robert J. F. Laheij, Peter C. Wever, Cornelis P.C. de Jager, J. Leuvenink, Mirrian Hilbink, Eugenie F.A. Gemen, Tom van der Poll |
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| Přispěvatelé: | Amsterdam institute for Infection and Immunity, Infectious diseases, Center of Experimental and Molecular Medicine |
| Rok vydání: | 2013 |
| Předmět: |
Male
Bacterial Diseases Critical Care and Emergency Medicine Pulmonology Epidemiology Lymphocyte lcsh:Medicine Lymphocyte Activation Leukocyte Count Immunophenotyping Molecular Cell Biology Cytotoxic T cell lcsh:Science Immune Response Multidisciplinary biology Middle Aged Flow Cytometry Lower Respiratory Tract Infections C-Reactive Protein Infectious Diseases medicine.anatomical_structure Medicine Female Legionnaires' Disease Research Article Adult Legionella Legionella pneumophila Immune system Respiratory Failure Antigen Antigens CD Lymphopenia medicine Humans Lymphocyte Count Biology Aged Legionellosis business.industry lcsh:R C-reactive protein medicine.disease Lymphocyte Subsets Biomarker Epidemiology Peripheral blood lymphocyte Respiratory Infections Immunology biology.protein lcsh:Q Clinical Immunology Lymphocytopenia business Cytometry |
| Zdroj: | PLoS ONE PLoS ONE, 8(4). Public Library of Science PLoS ONE, Vol 8, Iss 4, p e62265 (2013) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0062265 |
| Popis: | Study Objective: Absolute lymphocytopenia is recognised as an important hallmark of the immune response to severe infection and observed in patients with Legionnaires’ disease. To explore the immune response, we studied the dynamics of peripheral blood lymphocyte subpopulations in the acute and subacute phase of LD. Methods and Results: EDTA-anticoagulated blood was obtained from eight patients on the day the diagnosis was made through detection of L. pneumophila serogroup 1 antigen in urine. A second blood sample was obtained in the subacute phase. Multiparametric flow cytometry was used to calculate lymphocyte counts and values for B-cells, T-cells, NK cells, CD4 + and CD8 + T-cells. Expression of activation markers was analysed. The values obtained in the subacute phase were compared with an age and gender matched control group. Absolute lymphocyte count (610 9 /l, median and range) significantly increased from 0.8 (0.4–1.6) in the acute phase to 1.4 (0.8–3.4) in the subacute phase. B-cell count showed no significant change, while T-cell count (610 6 /l, median and range) significantly increased in the subacute phase (495 (182– 1024) versus 979 (507–2708), p = 0.012) as a result of significant increases in both CD4 + and CD8 + T-cell counts (374 (146– 629) versus 763 (400–1507), p = 0.012 and 119 (29–328) versus 224 (107–862), p = 0.012). In the subacute phase of LD, significant increases were observed in absolute counts of activated CD4 + T-cells, nao ¨ve CD4 + T-cells and memory CD4 + Tcells. In the CD8 + T-cell compartment, activated CD8 + T-cells, nao ¨ve CD8 + T-cell and memory CD8 + T-cells were significantly increased (p,0.05). Conclusion: The acute phase of LD is characterized by absolute lymphocytopenia, which recovers in the subacute phase with an increase in absolute T-cells and re-emergence of activated CD4 + and CD8 + T cells. These observations are in line with the suggested role for T-cell activation in the immune response to LD. |
| Databáze: | OpenAIRE |
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