Overexpression of long non-coding RNA LOC400891 promotes tumor progression and poor prognosis in prostate cancer
Autor: | Haiwei Yang, Jun Wang, Zengjun Wang, Chao Qin, Gong Cheng, Yongsheng Pan, Lixin Hua, Xiao Li |
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Rok vydání: | 2016 |
Předmět: |
Male
0301 basic medicine Stromal cell Bioinformatics Disease-Free Survival Cell Line Metastasis 03 medical and health sciences Prostate cancer 0302 clinical medicine Cell Movement Prostate Cell Line Tumor Biomarkers Tumor medicine Humans Neoplasm Invasiveness Aged Cell Proliferation Gene knockdown business.industry Prostatic Neoplasms General Medicine Prognosis medicine.disease Long non-coding RNA Gene Expression Regulation Neoplastic 030104 developmental biology medicine.anatomical_structure Tumor progression 030220 oncology & carcinogenesis Disease Progression Cancer research RNA Long Noncoding Neoplasm Recurrence Local business Immortalised cell line |
Zdroj: | Tumor Biology. 37:9603-9613 |
ISSN: | 1423-0380 1010-4283 |
Popis: | Tumor recurrence and metastasis remain the major obstacles for the successful treatment of patients diagnosed with prostate cancer (PCa). In recent years, long non-coding RNAs (lncRNAs) have been considered as key regulators of tumor behavior. In this study, we investigated the biological role and clinical relevance of the lncRNA LOC400891 in prostate cancer. Using of lncRNAs expression chips screening and the biological analysis, we found the target lncRNA (LOC400891). Moreover, the expression levels of lncRNA LOC400891 in PCa tissues and cell lines were evaluated by quantitative real-time PCR (qRT-PCR), and its association with biochemical recurrence-free survival of patients was analyzed by statistical analysis. Furthermore, the effect of LOC400891 on proliferation, migration, and invasion was studied in PCa cells. We found that the expression level of LOC400891 was higher in PCa tissues and cells compared to adjacent non-tumor tissues and normal prostate stromal immortalized cells WPMY-1. The patients with higher LOC400891 expression had an advanced clinical features and a shorter biochemical recurrence-free survival time than those with lower LOC400891 expression. Furthermore, multivariate analysis showed that the status of LOC400891 expression was an independent predictor of biochemical recurrence-free survival in PCa. We also found that knockdown of LOC400891 could inhibit cell proliferation, migration, and invasion in vitro study. Our data suggested that lncRNA LOC400891 was a novel molecule involved in PCa progression, which provided a potential prognostic biomarker and therapeutic target. |
Databáze: | OpenAIRE |
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