Enhanced Stability of Non-Canonical NPC2 in the symbiosome supports coral-algal symbiosis
| Autor: | Annika Guse, vaskoff Dk, Timo Sachsenheimer, Elizabeth A. Hambleton, Victor Arnold Shivas Jones, Ira Maegele |
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| Rok vydání: | 2018 |
| Předmět: |
0303 health sciences
biology Chemistry Host (biology) Coral fungi biochemical phenomena metabolism and nutrition biology.organism_classification Sterol Cell biology 03 medical and health sciences 0302 clinical medicine Symbiosome Symbiosis Organelle lipids (amino acids peptides and proteins) 14. Life underwater Aiptasia 030217 neurology & neurosurgery Function (biology) 030304 developmental biology |
| Popis: | Cnidarians such as reef-building corals depend upon nutrient transfer from intracellular symbionts, but the mechanisms and evolution of this process remain unknown. Homologues of the conserved cholesterol binder Niemann-Pick Type C2 (NPC2) in cnidarians are implicated in the transfer of sterol from symbionts. Here, we show that symbionts transfer bulk sterols to the host, host sterol utilization is plastic, and pharmacological inhibition of sterol trafficking disrupts symbiosis. Having undergone an anthozoan-specific expansion, “non-canonical” NPC2s respond to symbiosis and accumulate over time at the lysosomal-like organelle in which the symbiont resides (“symbiosome”). We demonstrate that both a non- and canonicalAiptasiaNPC2 bind symbiont-produced sterols, yet only the non-canonical homologue exhibits increased stability at low pH. We propose that symbiotic cnidarians adapted pre-existing cholesterol-trafficking machinery to function in the highly acidic symbiosome environment, allowing corals to dominate nutrient-poor shallow tropical seas worldwide. |
| Databáze: | OpenAIRE |
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