Mitochondrial D-loop nucleotide diversity in Astyanax (Osteichthyes, Characidae) from the upper Paraná and upper Paraguay River basins
| Autor: | T.V. Soria, Alberto José Prioli, V. N. Gomes, V.A. Carlo, Carla Simone Pavanelli, R.A. Prioli, L. M. Prioli, H. F. Júlio, Sônia Maria Alves Pinto Prioli |
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| Rok vydání: | 2012 |
| Předmět: |
Species complex
Floodplain Molecular Sequence Data Drainage basin Zoology Structural basin DNA Mitochondrial Haplogroup Nucleotide diversity Evolution Molecular D-loop Sequence Homology Nucleic Acid Genetics Animals Molecular Biology DNA Primers geography geography.geographical_feature_category Polymorphism Genetic biology Base Sequence Characidae General Medicine biology.organism_classification Haplotypes Brazil |
| Zdroj: | Genetics and molecular research : GMR. 11(2) |
| ISSN: | 1676-5680 |
| Popis: | The morphological discrimination between the species Astyanax altiparanae and A. asuncionensis of the upper Parana River and Paraguay River basins, respectively, has always been difficult. Two D-loop haplogroups of A. altiparanae are known, one with the presence (AltoPR) or the absence (AltoPR-D) of a 32-bp block similar to that in A. asuncionensis. We examined these samples to characterize A. altiparanae and verify whether A. asuncionensis occurred in the upper Parana River prior to the submergence of the Sete Quedas Falls when Itaipu reservoir was impounded. D-loop sequences were analyzed in A. altiparanae of the upper Parana and Iguacu Rivers and those of A. asuncionensis of the upper Paraguay River. The haplogroup AltoPR was found at all sites of the upper Parana and Iguacu Rivers, whereas AltoPR-D occurred in the Itaipu reservoir, floodplain and in the Tiete and Grande Rivers. Two haplogroups of A. asuncionensis were identified and both did not have the 32-bp block. However, AltoPR and AltoPR-D differed from one another in 5.1% of their bases and between 8.9 and 12.5% with regard to the haplogroups of the upper Paraguay basin. Further, AltoPR-D occurred in the Grande River upstream the Marimbondo Falls and other older reservoirs than Itaipu. The results reject the hypothesis of the establishment of A. asuncionensis and suggest that the haplogroup AltoPR-D existed in the upper Parana River before the impounding of the Itaipu reservoir. Moreover, morphological similarity and high genetic variation within the altiparanae/asuncionensis group suggest the existence of a cryptic species complex. |
| Databáze: | OpenAIRE |
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