Direct identification from Bact/Alert™ blood culture bottles by MALDI-TOF
| Autor: | Giacomo Fortina, Paola Macaluso, Marcella Perone, Vesselina Kroumova, Monia Mantovani, Tommaso Giani, Elisa Gobbato |
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| Rok vydání: | 2011 |
| Předmět: |
medicine.diagnostic_test
medicine.drug_class Bact alert Antibiotics lcsh:QR1-502 General Medicine Biology biology.organism_classification lcsh:Microbiology Microbiology Blood culture bottles medicine Identification (biology) Blood culture Bacterial identification blood culture mass spectrometry Pathogen Bacteria Gram |
| Zdroj: | Microbiologia Medica, Vol 26, Iss 4 (2011) |
| ISSN: | 2280-6423 |
| Popis: | Bacterial identification from blood culture using traditional methods needs about 48 hours, since positivization, to be performed. Rapid bacterial identification can result in clinical and economic benefits. To provide rapid pathogen identification for targeted antibiotic treatment, in this study we tested an our previously described homemade method for bacterial identification using MALDI-TOF directly from positive BACTEC blood culture, on positive BacT/ALERT blood culture. A total of 108 bacteria were identified by MALDI-TOF with a positive identification obtained for 98% of Gram negative and 84,3% of Gram positive bacteria.The average of identification score obtained using the protocol described in this study was 2,047 for Gram positive and 2,204 for Gram negative microorganisms. Data here described show that this method is also useful when BacT/ALERT bottles are used and even if these bottles have activated charcoal as inhibitor of antibiotics. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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