A new, versatile field immunosensor for environmental pollutantsDevelopment and proof of principle with TNT, diuron, and atrazine
| Autor: | David Tiemann, Gunther Kolb, Petra M. Krämer, Ines Frese, Stefan Windisch, Cristina M. Weber, Antonius Kettrup, Ioan M. Ciumasu |
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| Rok vydání: | 2005 |
| Předmět: |
Analyte
Biomedical Engineering Biophysics Enzyme-Linked Immunosorbent Assay Biosensing Techniques Signal law.invention chemistry.chemical_compound law Electrochemistry Atrazine Chemiluminescence Detection limit Chromatography Equipment Design General Medicine Microfluidic Analytical Techniques Chip Equipment Failure Analysis chemistry Proof of concept Diuron Flow Injection Analysis Feasibility Studies Environmental Pollution Luminescence Environmental Monitoring Trinitrotoluene Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 21:354-364 |
| ISSN: | 0956-5663 |
| Popis: | This paper presents a new, versatile, portable miniaturized flow-injection immunosensor which is designed for field analysis. The temperature-controlled field prototype can run for 6 h without external power supply. The bio-recognition element is an analyte-specific antibody immobilized on a gold surface of pyramidal structures inside an exchangeable single-use chip, which hosts also the enzyme-tracer and the sample reservoirs. The competition between the enzyme-tracer and the analyte for the antigen-binding sites of the antibodies yields in the final step a chemiluminescence signal that is inversely proportional to the concentration of analyte in the given range of detection. A proof of principle is shown for nitroaromatics and pesticides. The detection limits (DL; IC 20 ) reached with the field prototype in the laboratory was below 0.1 μg l −1 for 2,4,6-trinitrotoluene (TNT), and about 0.2 μg l −1 for diuron and atrazine, respectively. Important aspects in this development were the design of the competition between analyte and enzyme-tracer, the unspecific signal due to unspecific binding and/or luminescence background signal, and the flow pattern inside the chip. |
| Databáze: | OpenAIRE |
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