Genetic Analysis of Hepatitis A Virus Strains That Induced Epidemics in Korea during 2007–2009
| Autor: | Heasun Yun, Doo-Sung Cheon, Jong-Hyun Kim, Kisang Kim, Jai Myung Yang, Hyeokjin Lee, Hyesook Jeong |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Male
Microbiology (medical) Genotype Sequence analysis Molecular Sequence Data Biology Genetic analysis Virology Republic of Korea Humans Amino Acid Sequence Epidemics Genotyping Phylogeny Viral Structural Proteins Sequence Analysis RNA Outbreak Hepatitis A Industrial region Hepatitis a virus Molecular Typing Amino Acid Substitution biology.protein Female Antibody Hepatitis A Virus Human |
| Zdroj: | Journal of Clinical Microbiology. 50:1252-1257 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.01114-11 |
| Popis: | Hepatitis A virus is one of the most prominent causes of fecally transmitted acute hepatitis worldwide. In order to characterize the viral agents causing an outbreak in Korea (comprising North and South Korea) from June 2007 to May 2009, we collected specimens and performed genotyping of the VP1/P2A and VP3/VP1 regions of hepatitis A virus. We then used a multiple-alignment algorithm to compare the nucleotide sequences of the 2 regions with those of reference strains. Hepatitis A virus antibodies were detected in 64 patients from 5 reported outbreaks (North Korea, June 2007 [ n = 11]; Jeonnam, April 2008 [ n = 15]; Daegu, May 2008 [ n = 13]; Seoul, May 2009 [ n = 22]; and Incheon, May 2009 [ n = 3]). We found 100% homology between strains isolated from the Kaesong Industrial Region and Jeonnam. While those strains were classified as genotype IA strains, strains from Seoul and Incheon were identified as genotype IIIA strains and showed 98.9 to 100% homology. Genotype IIIA was also dominant in Daegu, where strains were 95.7 to 100% homologous. All hepatitis A virus strains isolated from the Kaesong Industrial Region, Jeonnam, Seoul, and Incheon belonged to a single cluster. However, strains from Daegu could be classified into 2 clusters, suggesting that the outbreak had multiple sources. This study indicates that hepatitis A virus strains of 2 different genotypes are currently cocirculating in Korea. Moreover, it documents an increasing prevalence of genotype IIIA strains in the country. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|