Multimodal optical coherence tomography and fluorescence lifetime imaging with interleaved excitation sources for simultaneous endogenous and exogenous fluorescence
| Autor: | Xi Chen, Jesung Park, Brian E. Applegate, Brian L. Walton, Michael J. Serafino, Siqin Zhaorigetu, Javier A. Jo, Jesus Rico-Jimenez, Sebina Shrestha |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Fluorescence-lifetime imaging microscopy Materials science Ghost imaging 01 natural sciences Article law.invention 010309 optics 03 medical and health sciences Optics Optical coherence tomography law 0103 physical sciences medicine Fluorescence microscope medicine.diagnostic_test business.industry Laser Fluorescence Atomic and Molecular Physics and Optics 030104 developmental biology business Preclinical imaging Excitation Biotechnology Biomedical engineering |
| Popis: | Multimodal imaging probes a variety of tissue properties in a single image acquisition by merging complimentary imaging technologies. Exploiting synergies amongst the data, algorithms can be developed that lead to better tissue characterization than could be accomplished by the constituent imaging modalities taken alone. The combination of optical coherence tomography (OCT) with fluorescence lifetime imaging microscopy (FLIM) provides access to detailed tissue morphology and local biochemistry. The optical system described here merges 1310 nm swept-source OCT with time-domain FLIM having excitation at 355 and 532 nm. The pulses from 355 and 532 nm lasers have been interleaved to enable simultaneous acquisition of endogenous and exogenous fluorescence signals, respectively. The multimodal imaging system was validated using tissue phantoms. Nonspecific tagging with Alexa Flour 532 in a Watanbe rabbit aorta and active tagging of the LOX-1 receptor in human coronary artery, demonstrate the capacity of the system for simultaneous acquisition of OCT, endogenous FLIM, and exogenous FLIM in tissues. |
| Databáze: | OpenAIRE |
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