DNM1, a Dynamin-Related Protein That Contributes to Endocytosis and Peroxisome Fission, Is Required for the Vegetative Growth, Sporulation, and Virulence of Metarhizium robertsii
| Autor: | Zhenbang Liu, Yulong Wang, Rui Xie, Xiangyun Xie, Deshui Yu, Bo Huang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Dynamins
Metarhizium Mutant Virulence Conidiation Moths Biology Endocytosis Applied Microbiology and Biotechnology Fungal Proteins 03 medical and health sciences Gene Expression Regulation Fungal Environmental Microbiology Peroxisomes Animals Peroxisome fission Pest Control Biological 030304 developmental biology Dynamin 0303 health sciences Ecology 030306 microbiology fungi Spores Fungal Peroxisome Cell biology Complementation Food Science Biotechnology |
| Zdroj: | Appl Environ Microbiol |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.01217-20 |
| Popis: | Although dynamins and dynamin-related proteins (DRPs), a large GTPase superfamily, are involved in the budding of transport vesicles and division of organelles in eukaryotic cells, the function of these proteins in entomopathogenic fungi has not been reported to date. Here, DNM1, a DRP in Metarhizium robertsii, was characterized using gene disruption and complementation strategies. Mutant phenotype assays showed that the ΔDnm1 strain displayed increased defects in radial growth (∼24%) and conidial production (∼42%) compared to those of the wild type (WT), and reduced conidiation levels were accompanied by the repression of several key conidiation-related genes, including flbA, wetA, and flbD. Additionally, mutant bioassays revealed that disruption of Dnm1 impaired the virulence (both topical inoculation and injection) of M. robertsii in the insect Galleria mellonella. Further analysis demonstrated that deleting Dnm1 in fungi suppressed the transcriptional levels of several virulence genes in the insect hemocoel. Moreover, we found that DNM1 colocalized with peroxisomes and mitochondria. Importantly, disruption of Dnm1 abolished normal fungal endocytosis, resulting in significantly decreased numbers of, as well as morphological changes in, peroxisomes. These findings indicate that deletion of Dnm1 causes significant changes in the vegetative growth, sporulation, and virulence of M. robertsii due to changes in cell function and peroxisomes. IMPORTANCE Dnm1 was found to be involved in fungal development and virulence, mediated peroxisomal fission, and normal endocytosis. This finding provides new insights into the cellular processes and pathogenicity in entomopathogenic fungi. |
| Databáze: | OpenAIRE |
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