Genetic mapping and QTL analysis for peanut smut resistance
Autor: | Alicia N. Massa, Cecilia Bruno, Mario I. Buteler, Sara J. Soave, Claudio Oddino, Marina Bressano, Francisco J. de Blas, Melina Rosso, Renee S. Arias, Eva Mamani, J. Guillermo Seijo, Juan H. Soave, Beatriz P. Costero, Carolina Ballén-Taborda |
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Rok vydání: | 2021 |
Předmět: |
Genetic Markers
0106 biological sciences 0301 basic medicine Arachis Introgression QTL Quantitative Trait Loci Population Plant Science Plant disease resistance Quantitative trait locus Polymorphism Single Nucleotide 01 natural sciences RIL population 03 medical and health sciences Peanut smut Inbreeding education Genetic Association Studies Plant Diseases Recombination Genetic Genetics Disease resistance education.field_of_study biology Hypogaea Botany food and beverages Chromosome Mapping biology.organism_classification Genetic architecture Groundnut Peanut Phenotype 030104 developmental biology Genetic distance QK1-989 Smut Research Article 010606 plant biology & botany |
Zdroj: | BMC Plant Biology BMC Plant Biology, Vol 21, Iss 1, Pp 1-15 (2021) |
ISSN: | 1471-2229 |
Popis: | Background Peanut smut is a disease caused by the fungus Thecaphora frezii Carranza & Lindquist to which most commercial cultivars in South America are highly susceptible. It is responsible for severely decreased yield and no effective chemical treatment is available to date. However, smut resistance has been identified in wild Arachis species and further transferred to peanut elite cultivars. To identify the genome regions conferring smut resistance within a tetraploid genetic background, this study evaluated a RIL population {susceptible Arachis hypogaea subsp. hypogaea (JS17304-7-B) × resistant synthetic amphidiploid (JS1806) [A. correntina (K 11905) × A. cardenasii (KSSc 36015)] × A. batizocoi (K 9484)4×} segregating for the trait. Results A SNP based genetic map arranged into 21 linkage groups belonging to the 20 peanut chromosomes was constructed with 1819 markers, spanning a genetic distance of 2531.81 cM. Two consistent quantitative trait loci (QTLs) were identified qSmIA08 and qSmIA02/B02, located on chromosome A08 and A02/B02, respectively. The QTL qSmIA08 at 15.20 cM/5.03 Mbp explained 17.53% of the phenotypic variance, while qSmIA02/B02 at 4.0 cM/3.56 Mbp explained 9.06% of the phenotypic variance. The combined genotypic effects of both QTLs reduced smut incidence by 57% and were stable over the 3 years of evaluation. The genome regions containing the QTLs are rich in genes encoding proteins involved in plant defense, providing new insights into the genetic architecture of peanut smut resistance. Conclusions A major QTL and a minor QTL identified in this study provide new insights into the genetic architecture of peanut smut resistance that may aid in breeding new varieties resistant to peanut smut. |
Databáze: | OpenAIRE |
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