Tyrosinase/Chitosan/Reduced Graphene Oxide Modified Screen-Printed Carbon Electrode for Sensitive and Interference-Free Detection of Dopamine
| Autor: | Jui Hsuan Tsai, Yi Chun Yeh, Cheng You Liu, Jian-Zhang Chen, Yi Chieh Chou, Tzu Ming Huang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Tyrosinase
02 engineering and technology tyrosinase 010402 general chemistry 01 natural sciences reduced graphene oxide lcsh:Technology law.invention Chitosan lcsh:Chemistry chemistry.chemical_compound law General Materials Science Instrumentation lcsh:QH301-705.5 Fluid Flow and Transfer Processes Detection limit Graphene lcsh:T Process Chemistry and Technology General Engineering screen printing 021001 nanoscience & nanotechnology Ascorbic acid lcsh:QC1-999 0104 chemical sciences Computer Science Applications electrochemical biosensor chemistry lcsh:Biology (General) lcsh:QD1-999 lcsh:TA1-2040 Electrode Screen printing Cyclic voltammetry chitosan dopamine 0210 nano-technology lcsh:Engineering (General). Civil engineering (General) lcsh:Physics Nuclear chemistry |
| Zdroj: | Applied Sciences, Vol 9, Iss 4, p 622 (2019) Applied Sciences Volume 9 Issue 4 |
| ISSN: | 2076-3417 |
| Popis: | Tyrosinase, chitosan, and reduced graphene oxide (rGO) are sequentially used to modify a screen-printed carbon electrode (SPCE) for the detection of dopamine (DA), without interference from uric acid (UA) or ascorbic acid (AA). The use of tyrosinase significantly improves the detection&rsquo s specificity. Cyclic voltammetry (CV) measurements demonstrate the high sensitivity and selectivity of the proposed electrochemical sensors, with detection limits of 22 nM and broad linear ranges of 0.4&ndash 8 &mu M and 40&ndash 500 &mu M. The fabricated tyrosinase/chitosan/rGO/SPCE electrodes achieve satisfactory results when applied to human urine samples, thereby demonstrating their feasibility for analyzing DA in physiological samples. |
| Databáze: | OpenAIRE |
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