Effects of insulin-like growth factor-1 on random pattern skin flap survival in rats
| Autor: | Nurten Turhan Haktanir, Yavuz Demir, Güray Yılmaz, Mehmet Fatih Bozkurt |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Survival RD1-811 medicine.medical_treatment Injections Subcutaneous Skin flap Urology Anti-Inflammatory Agents Surgical Flaps Rats Sprague-Dawley 03 medical and health sciences Insulin-like growth factor chemistry.chemical_compound 0302 clinical medicine Random pattern medicine Flap survival Animals Insulin-Like Growth Factor I Saline Skin Wound Healing business.industry Vascular Endothelial Growth Factors Significant difference Graft Survival Surgery Rats Vascular endothelial growth factor 030104 developmental biology chemistry 030220 oncology & carcinogenesis business Wound healing |
| Zdroj: | Acta Cirurgica Brasileira, Vol 31, Iss 8, Pp 513-519 (2016) Acta Cirúrgica Brasileira v.31 n.8 2016 Acta Cirúrgica Brasileira Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC Acta Cirurgica Brasileira, Volume: 31, Issue: 8, Pages: 513-519, Published: AUG 2016 |
| ISSN: | 1678-2674 |
| Popis: | PURPOSE: To investigate the effects of pharmacological delay with insulin-like growth factor-1 (IGF-1) on skin flap survival. METHODS: Thirty Sprague-Dawley rats were submitted to dorsal skin flap (3x9 cm). Seven days before the surgery, the animals were subdivided into three groups of 10 rats. In group 1 (controls), no injection was done. Seven days before the elevation, saline had been injected to the marked skin flap area in group 2 (sham group), and group 3 (experimental group) underwent a pharmacological delay with subcutaneous IGF-1 injections. On the seventh postoperative day, flap area was analyzed for survival. Tissue samples were obtained for histological and biochemical evaluations. RESULTS: Survival rates were 43.55 ± 16%, 21.40 ± 8%, and 43.12 ± 14% in groups 1, 2, and 3, respectively. Differences between group 2 and other groups were statistically significant. No significant difference was detected between all three groups for tissue or plasma vascular endothelial growth factor (VEGF) levels. There was no significant histological difference between groups. CONCLUSION: Although a single injection of insulin-like growth factor-1 (IGF-1) did not significantly increase flap survival, its wound healing features are still encouraging and further meticulously planned studies, especially with repeated applications or controlled-release methods, and combinations with binding protein are required. |
| Databáze: | OpenAIRE |
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