Cellular compartmentalization of internalized paramagnetic liposomes strongly influences both T(1) and T(2) relaxivity
| Autor: | Maarten B. Kok, Willem J. M. Mulder, Daisy W. J. van der Schaft, Sjoerd Hak, Gustav J. Strijkers, Klaas Nicolay |
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| Přispěvatelé: | Medical Biochemistry, Other departments, Soft Tissue Biomech. & Tissue Eng. |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
media_common.quotation_subject
Integrin Molecular Probe Techniques Sensitivity and Specificity Cell membrane Magnetics Nuclear magnetic resonance Cell surface receptor In vivo medicine Humans Radiology Nuclear Medicine and imaging Internalization Beta (finance) Receptor Cells Cultured media_common Liposome biology Chemistry Endothelial Cells Reproducibility of Results Magnetic Resonance Imaging medicine.anatomical_structure Liposomes biology.protein Biophysics Subcellular Fractions |
| Zdroj: | Magnetic resonance in medicine, 61(5), 1022-1032. John Wiley and Sons Inc. Magnetic Resonance in Medicine, 61(5), 1022-1032. Wiley |
| ISSN: | 0740-3194 |
| DOI: | 10.1002/mrm.21910 |
| Popis: | In recent years, numerous Gd(3+)-based contrast agents have been developed to enable target-specific MR imaging of in vivo processes at the molecular level. The combination of powerful contrast agents and amplification strategies, aimed at increasing the contrast agent dose at the target site, is an often-used strategy to improve the sensitivity of biomarker detection. One such amplification mechanism is to target a disease-specific cell membrane receptor that can undergo multiple rounds of internalization following ligand binding and thus shuttle a sizeable amount of contrast agent into the target cell. An example of such a membrane receptor is the alpha(nu)beta(3) integrin. The goal of this study was to investigate the consequences of this amplification approach for the T(1)- and T(2)-shortening efficacy of a paramagnetic contrast agent. Cultured endothelial cells were incubated with paramagnetic liposomes that were conjugated with a cyclic RGD-peptide to enable internalization by means of the alpha(nu)beta(3) integrin receptor. Non-targeted liposomes served as a control. This study showed that alpha(nu)beta(3) targeting dramatically increased the uptake of paramagnetic liposomes. This targeting strategy, however, strongly influenced both the longitudinal and transverse relaxivity of the internalized paramagnetic liposomes. |
| Databáze: | OpenAIRE |
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