Synthesis and biological evaluation of mouse growth hormone-releasing factor
| Autor: | Mushtaq Ahmad, Thomas R. Downs, Edgar P. Heimer, Theodore Lambros, Lawrence A. Frohman, Arthur Felix |
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| Rok vydání: | 2009 |
| Předmět: |
Male
medicine.medical_specialty Somatotropic cell Ratón medicine.medical_treatment Molecular Sequence Data Peptide Growth Hormone-Releasing Hormone Biochemistry Mice chemistry.chemical_compound Anterior pituitary Pituitary Gland Anterior Internal medicine Peptide synthesis medicine Animals Humans Amino Acid Sequence Cells Cultured chemistry.chemical_classification Growth factor Rats Inbred Strains Biological activity Rats Pituicyte Endocrinology medicine.anatomical_structure chemistry Growth Hormone |
| Zdroj: | International Journal of Peptide and Protein Research. 37:552-555 |
| ISSN: | 0367-8377 |
| Popis: | The recently described mouse growth hormone-releasing factor (mGRF) was synthesized by the solid phase procedure, purified by 2 stages of preparative high performance liquid chromatography and fully characterized. The biologic activity of the 42-amino acid peptide (H-His-Val-Asp-Ala-Ile-Phe- Thr-Thr-Asn-Tyr- Arg-Lys-Leu-Leu-Ser-Gln-Leu-Tyr-Ala-Arg-Lys-Val-Ile-Gln-Asp-Ile-Met-Asn- Lys- Gln-Gly-Glu-Arg-Ile- Gln-Glu-Gln-Arg-Ala-Arg-Leu-Ser-OH) was assessed in primary cultures of both mouse and rat anterior pituitary cells and compared to synthetic rat (rGRF) and human (hGRF) growth hormone-releasing factors. mGRF was equipotent to rGRF in mouse somatotrophs but slightly less potent in rat somatotrophs, while hGRF was 3-5 times less potent in both rodent species. |
| Databáze: | OpenAIRE |
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