In vivo release and retinal safety of intravitreal implants of thalidomide in rabbit eyes and antiangiogenic effect on the chorioallantoic membrane

Autor:	Murilo M O Miranda, Antonio Haddad, Sílvia Ligório Fialho, Pedro A F Souza, Rodrigo Jorge, Armando Silva-Cunha, Andre Messias, Bruno Gonçalves Pereira, Gustavo de Oliveira Fulgêncio
Rok vydání:	2013
Předmět:	Pharmaceutical Science Angiogenesis Inhibitors Chick Embryo Pharmacology Chorioallantoic Membrane Retina chemistry.chemical_compound In vivo medicine Electroretinography Animals Drug Implants medicine.diagnostic_test business.industry Retinal Controlled release Thalidomide Vitreous Body PLGA Chorioallantoic membrane chemistry Solubility Toxicity Female Rabbits business medicine.drug
Zdroj:	Journal of drug targeting. 21(9)
ISSN:	1029-2330
Popis:	To evaluate the in vivo release, retinal safety and antiangiogenic effect of a thalidomide-loaded poly-lactide-co-glycolide intravitreal implant.New Zealand white rabbits, divided into two groups, I and II, received an intravitreal implant containing or not thalidomide, respectively (n = 12). Intravitreal drug levels were determined during a six-week study period. The potential for toxicity associated with the implants was evaluated by electroretinography and light microscopy (n = 8). Twelve chorioallantoic membranes (CAMs) from chicken eggs were incubated with thalidomide dispersion, implants containing or not thalidomide and vitreous samples and analyzed after two days regarding the percentage of vessels regression.Intravitreal concentrations of thalidomide (ng/ml) were 690.21 ± 177.95, 372.51 ± 185.56, 240.59 ± 133.48, 327.54 ± 169.71, 294.26 ± 142.41 and 465.18 ± 157.51 at 1, 2, 3, 4, 5 and 6 weeks, respectively, after implantation in group I rabbits. No drug was detected in group II samples. Electroretinography and histological evaluations did not show any sign of retina toxicity. There was significant regression of vessels in CAM incubated with thalidomide dispersion, thalidomide-loaded implants and vitreous samples from group I when compared to control.The intravitreal implants delivered safe doses of thalidomide that were also effective to induce vessels regression in CAMs.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1a95e916a59bdd3497cacbabf84e4ff9 https://pubmed.ncbi.nlm.nih.gov/23952807 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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