Elite model for the generation of induced pluripotent cancer cells (iPCs)

Autor: Dashayini Mahalingam, Chiou Mee Kong, Xiaojin Xie, Xueying Wang, Jason Kuan Han Lai
Rok vydání: 2012
Předmět:
Somatic cell
lcsh:Medicine
Mice
SCID
medicine.disease_cause
Mice
Molecular Cell Biology
Basic Cancer Research
Induced pluripotent stem cell
lcsh:Science
Genetics
Mutation
education.field_of_study
Multidisciplinary
Reverse Transcriptase Polymerase Chain Reaction
Stem Cells
Cancer Risk Factors
Cellular Reprogramming
Gene Expression Regulation
Neoplastic
Oncology
Neoplastic Stem Cells
Medicine
Cellular Types
Reprogramming
Research Article
Pluripotent Stem Cells
Evolutionary Processes
Population
Blotting
Western
Induced Pluripotent Stem Cells
Molecular Sequence Data
Transplantation
Heterologous
Genetic Causes of Cancer
Mice
Nude
Tumor cells
Computational biology
Biology
Cell Line
Cell Line
Tumor
medicine
Cancer Genetics
Animals
Humans
education
Cyclin-Dependent Kinase Inhibitor p16
Cyclin-Dependent Kinase Inhibitor p15
Evolutionary Biology
Models
Genetic
lcsh:R
Neoplasms
Experimental
Sequence Analysis
DNA
Cell culture
Cancer cell
lcsh:Q
Tumor Suppressor Protein p53
HeLa Cells
Developmental Biology
Zdroj: PLoS ONE
PLoS ONE, Vol 8, Iss 2, p e56702 (2013)
ISSN: 1932-6203
Popis: The inefficiency of generating induced pluripotent somatic cells (iPS) engendered two contending models, namely the Stochastic model and Elite model. Although the former is more favorable to explain the inherent inefficiencies, it may be fallible to extrapolate the same working model to reprogramming of cancer cells. Indeed, tumor cells are known to be inherently heterogeneous with respect to distinctive characteristics thus providing a suitable platform to test whether the reprogramming process of cancer cells is biased. Here, we report our observations that all randomly picked induced pluripotent cancer cells (iPCs) established previously do not possess mutations known in the parental population. This unanticipated observation is most parsimoniously explained by the Elite model, whereby putative early tumor progenies were selected during induction to pluripotency.
Databáze: OpenAIRE
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