Mitochondrial genes associated with pyrethroid resistance revealed by mitochondrial genome and transcriptome analyses in the malaria vector Anopheles sinensis (Diptera: Culicidae)
| Autor: | Bin Chen, Sana Asghar, Zhen-Tian Yan, Xu-Dong Li, Yi-Ran Ding, Qi-Meng Mao, Feng-Ling Si |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Mitochondrial DNA China Insecticides ved/biology.organism_classification_rank.species Respiratory chain Population genetics Mosquito Vectors Biology 01 natural sciences Nucleotide diversity Transcriptome Anopheles sinensis Insecticide Resistance chemistry.chemical_compound parasitic diseases Anopheles Pyrethrins Animals Gene Genetics Pyrethroid ved/biology Gene Expression Profiling General Medicine Malaria 010602 entomology Genes Mitochondrial chemistry Insect Science Genome Mitochondrial Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Pest management scienceREFERENCES. 76(2) |
| ISSN: | 1526-4998 |
| Popis: | Background Insecticides are still the main method of mosquito control, but mosquito resistance presents a large obstacle. The function of mitochondrial genes in the evolution of insecticide resistance is still poorly understood. Pyrethroid is the most commonly used insecticide, and Anopheles sinensis is an important malaria vector in China and Southeast Asia. In this study, we investigated the mitochondrial genes associated with pyrethroid resistance through their genetic and expression variation based on analyses of transcriptomes and 36 individuals with resequencing in three geographical populations in China. Results The nucleotide diversity (Pi) in 18 resistant individuals was much lower than that in 18 susceptible individuals, which suggests that some sites experienced purifying selection subject to pyrethroid stress. Ka/Ks and amino acid analyses showed that ND4 experienced positive selection and had 23 amino acid mutations due to pyrethroid stress. These mutations might change the ND4 structure and function and thus alter the efficiency of the respiratory chain. ND5 was significantly upregulated, and ATP8 was significantly downregulated in these three pyrethroid resistant populations, which suggests that these two genes function in the production and maintenance of pyrethroid resistance. There are differences in mitochondrial genes involved in pyrethroid resistance among these three populations. Conclusion This is the first study to reveal the association of mitochondrial genes in the evolution of insecticide resistance through amino acid mutation and expression patterns and can help us further understand insecticide resistance mechanisms. © 2019 Society of Chemical Industry. |
| Databáze: | OpenAIRE |
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