Cloning and characterization of two chlorophyll A/B binding protein genes and analysis of their gene family in Camellia sinensis
Autor: | Chu-Yan Chen, Xiao-Nan Mao, Yu-Lin Zhu, Ting-Ting Ye, Fang Du, Xiang-Yong Li, Xian-Wen Li, Geng Zhijuan |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0106 biological sciences
0301 basic medicine Models Molecular Subfamily Plant molecular biology Protein Conformation lcsh:Medicine Biology Genes Plant 01 natural sciences Genome Article Camellia sinensis Photosystem II Transcriptome 03 medical and health sciences Structure-Activity Relationship Gene Expression Regulation Plant Gene family Cloning Molecular Photosynthesis lcsh:Science Transcriptomics Gene Phylogeny Cloning Regulation of gene expression Multidisciplinary Gene Expression Profiling lcsh:R fungi food and beverages Gene expression profiling 030104 developmental biology Biochemistry Plant stress responses Multigene Family lcsh:Q Chlorophyll Binding Proteins 010606 plant biology & botany |
Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-9 (2020) |
ISSN: | 2045-2322 |
Popis: | In this study, two chlorophyll A/B binding protein (CAB) genes (CsCP1 and CsCP2) in tea plant were cloned. The proteins encoded by these genes belong to the external or internal antenna proteins of PS II, respectively. They may be the targets of physiological regulation for tea leaf cell PS II because they all contain multiple functional domains and modifiable sites. The CAB gene family in the tea genome consists of 25 homologous genes. We measured the expression patterns of ten genes in the CsCP1 and CsCP2 subfamily under six different stresses. CsCP1 expression was inhibited in response to 6 kinds of stress; CsCP2 expression was slightly upregulated only after cold stress and ABA treatment. However, the expression levels of CSA016997 and CSA030476 were upregulated significantly in the six stresses. The results suggested that the 10 CAB genes may have different functions in tea leaves. Moreover, changes in the expression of the 10 genes under stress appear to be related to ABA- and MeJA-dependent signalling pathways, and their responses to MeJA treatment is faster than those to ABA. In addition, we introduced our experiences for cloning the genes in the context of complex genomes. |
Databáze: | OpenAIRE |
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