Upregulation of 15 Antisense Long Non-Coding RNAs in Osteosarcoma
| Autor: | Jiake Xu, David Wood, Aare Märtson, Xuan Dung Ho, Emel Rothzerg, Sulev Kõks |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
sarcoma non-coding RNA Bone Neoplasms QH426-470 Biology Article 03 medical and health sciences alternative splicing 0302 clinical medicine Transcription (biology) Cell Line Tumor osteosarcoma Genetics medicine Humans RNA Antisense Genetics (clinical) antisense RNA Regulation of gene expression Sequence Analysis RNA Alternative splicing RNA medicine.disease Non-coding RNA Antisense RNA Up-Regulation Gene Expression Regulation Neoplastic 030104 developmental biology 030220 oncology & carcinogenesis Cancer research Osteosarcoma Human genome RNA Long Noncoding |
| Zdroj: | Genes Volume 12 Issue 8 Genes, Vol 12, Iss 1132, p 1132 (2021) |
| ISSN: | 2073-4425 |
| DOI: | 10.3390/genes12081132 |
| Popis: | The human genome encodes thousands of natural antisense long noncoding RNAs (lncRNAs) they play the essential role in regulation of gene expression at multiple levels, including replication, transcription and translation. Dysregulation of antisense lncRNAs plays indispensable roles in numerous biological progress, such as tumour progression, metastasis and resistance to therapeutic agents. To date, there have been several studies analysing antisense lncRNAs expression profiles in cancer, but not enough to highlight the complexity of the disease. In this study, we investigated the expression patterns of antisense lncRNAs from osteosarcoma and healthy bone samples (24 tumour-16 bone samples) using RNA sequencing. We identified 15 antisense lncRNAs (RUSC1-AS1, TBX2-AS1, PTOV1-AS1, UBE2D3-AS1, ERCC8-AS1, ZMIZ1-AS1, RNF144A-AS1, RDH10-AS1, TRG-AS1, GSN-AS1, HMGA2-AS1, ZNF528-AS1, OTUD6B-AS1, COX10-AS1 and SLC16A1-AS1) that were upregulated in tumour samples compared to bone sample controls. Further, we performed real-time polymerase chain reaction (RT-qPCR) to validate the expressions of the antisense lncRNAs in 8 different osteosarcoma cell lines (SaOS-2, G-292, HOS, U2-OS, 143B, SJSA-1, MG-63, and MNNG/HOS) compared to hFOB (human osteoblast cell line). These differentially expressed IncRNAs can be considered biomarkers and potential therapeutic targets for osteosarcoma. |
| Databáze: | OpenAIRE |
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