Phytochemical screening, metabolite profiling and enhanced antimicrobial activities of microalgal crude extracts in co-application with silver nanoparticle
| Autor: | Siti Aisha Mohd Radzi, Hanaa Ali Hussein, Julius Yong Fu Siong, Nor Atikah Mohamed Zin, Desy Fitrya Syamsumir, Mohd Azmuddin Abdullah |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
lcsh:Biotechnology Silver nanoparticle Biomedical Engineering Solvent systems 010501 environmental sciences lcsh:Chemical technology lcsh:Technology 01 natural sciences 03 medical and health sciences Phytol chemistry.chemical_compound Metabolite profiling Co-application lcsh:TP248.13-248.65 Microalgae lcsh:TP1-1185 Food science Carotenoid 0105 earth and related environmental sciences chemistry.chemical_classification biology lcsh:T Renewable Energy Sustainability and the Environment Antimicrobial biology.organism_classification Eicosapentaenoic acid Chlorella 030104 developmental biology Phytochemical chemistry Food Science Biotechnology Polyunsaturated fatty acid |
| Zdroj: | Bioresources and Bioprocessing, Vol 7, Iss 1, Pp 1-17 (2020) |
| ISSN: | 2197-4365 |
| DOI: | 10.1186/s40643-020-00322-w |
| Popis: | BackgroundMicroalgae is one of the major sources of natural compounds with antimicrobial activity. The metabolite profiling of the extracts could identify the bioactive compounds based on methanol (MET), ethanol (ETH), chloroform (CHL), hexane (HEX) and water (W) solvent systems. The microalgal crude extracts in co-application with silver nanoparticles (AgNPs) had enhanced antimicrobial activity with potential to overcome the global problem of microbial antibiotic resistance.ResultsChlorellasp. exhibited the highest lipid,N. oculatathe highest total saturated fatty acids (TSFA), andT. suecicathe highest mono-unsaturated (MUFA) and poly-unsaturated fatty acids (PUFA). The highest carbohydrate, protein and total phenolics contents (TPCs) were attained byN. oculata. The highest total flavonoids contents (TFCs), and chlorophyllaandbwere inT. suecica, while comparable level of carotenoids were found in all species. For high-performance thin-layer chromatography (HPTLC) analyses, the eicosapentaenoic acid (EPA) with high peaks were detected inT. suecica-HEX andN. oculata-CHL; and β-carotene inChlorellasp.-ETH. The gas chromatography–mass spectrometry (GC–MS) analyses showed high 13-docosenamide (Z)- inT. suecica-HEX; phytol inN. oculata-HEX; and neophytadiene inChlorellasp.-ETH. The AgNPs–MCEs–MET and HEX at the 1.5:1 ratios exhibited strong activities againstBacillus subtilis,Streptococcus uberis, andSalmonellasp.; and the AgNPs–T. suecica-HEX and MET and AgNPs–Chlorellasp.-HEX at the 1.5:1 ratios exhibited activities againstKlebsiella pneumoniae.ConclusionDifferent bioactive components were detected in the MCEs based on the HPTLC and GC–MS analyses. Significant antimicrobial activities against the pathogenic microbes were demonstrated by the synergistic effects of the MCEs in co-application with the AgNPs. This could be beneficial in the fight against sensitive and multidrug-resistant bacteria. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink