Radioprotective Effects of Mitochondria-Targeted Antioxidant SkQR1
| Autor: | Denis S. Izumov, Nikolay I. Riabchenko, Fetisova Ek, Konstantin G. Lyamzaev, Varvara D. Cherepanynets, Roman I. Kireev, Igor I. Kireev, Boris V. Chernyak, Margarita M. Antoschina, Vladimir P. Skulachev |
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| Rok vydání: | 2015 |
| Předmět: |
Time Factors
Antioxidant Plastoquinone DNA damage medicine.medical_treatment Biophysics Radiation-Protective Agents Biology medicine.disease_cause Antioxidants chemistry.chemical_compound medicine Humans DNA Breaks Double-Stranded Radiology Nuclear Medicine and imaging Chromosome Aberrations chemistry.chemical_classification Phosphorylated Histone H2AX Reactive oxygen species Radiation Rhodamines Cell Cycle Mitochondria Cell biology Nuclear DNA Biochemistry chemistry Gamma Rays K562 Cells Reactive Oxygen Species DNA Oxidative stress K562 cells |
| Zdroj: | Radiation Research. 183:64-71 |
| ISSN: | 1938-5404 0033-7587 |
| DOI: | 10.1667/rr13708.1 |
| Popis: | We show here that mitochondria-targeted antioxidant composed of plastoquinone conjugated through hydrocarbon linker with cationic rhodamine 19 (SkQR1) protected against nuclear DNA damage induced by gamma radiation in K562 erythroleukemia cells. We also demonstrate that SkQR1 prevented the early (1 h postirradiation) accumulation of phosphorylated histone H2AX (γ-H2AX) an indicator of DNA double-strand break formation, as well as the radiation-induced increase in chromosomal aberrations. These data suggested that nuclear DNA damage induced by gamma radiation may be mediated by mitochondrial reactive oxygen species (ROS) production. We show that SkQR1 suppressed delayed accumulation of ROS 32 h after irradiation probably by inhibiting mitochondrial ROS-induced ROS release mechanisms. This suggests that mitochondria-targeted antioxidants may protect cells from the late consequences of radiation exposure related to delayed oxidative stress. We have previously reported that SkQRl is the substrate of multidrug resistance pump P-glycoproten (Pgp 170) and selectively protects Pgp 170-negative cells against oxidative stress. In line with this finding, we demonstrate here that SkQR1 did not protect Pgp170-positive K562 subline against DNA damage induced by gamma radiation. The selective radioprotection of normal Pgp 170-negative cells by mitochondria-targeted antioxidants could be a promising strategy to increase the efficiency of radiotherapy for multidrug-resistant tumors. |
| Databáze: | OpenAIRE |
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