Comparison of blood microsampling with DBS and conventional blood collection techniques used in a midazolam biostudy
Autor: | Guy Havard, Olivier Barrière, Brigitte Pellerin, Ann Lévesque, Sara Croteau, Sylvain Lachance, Dominique Anctil, Marie-Claude Théberge |
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Rok vydání: | 2016 |
Předmět: |
genetic structures
Midazolam Clinical Biochemistry Blood volume 030226 pharmacology & pharmacy 01 natural sciences Analytical Chemistry 03 medical and health sciences 0302 clinical medicine Tandem Mass Spectrometry medicine Humans Two sample General Pharmacology Toxicology and Pharmaceutics Finger prick Chromatography High Pressure Liquid Dried Blood Spot Testing Whole blood Blood Specimen Collection Venipuncture business.industry 010401 analytical chemistry Temperature General Medicine Blood collection Reference Standards 0104 chemical sciences Medical Laboratory Technology surgical procedures operative ROC Curve Anesthesia Area Under Curve business medicine.drug Half-Life |
Zdroj: | Bioanalysis. 8(8) |
ISSN: | 1757-6199 |
Popis: | Background: Quantitative DBS LC–MS/MS assay for midazolam was used to compare two sample collection techniques (venipuncture and finger prick) and the midazolam concentrations measured in plasma samples, DBS and dried plasma spots. Methodology: Midazolam was extracted from DBS cards and compared with whole blood collected from usual venipuncture. Dried plasma spots were also compared with plasma. The blood volume used as well as the temperature impact during the blood and plasma deposits was evaluated. Midazolam was administrated to six healthy subjects during a clinical trial to obtained blood and plasma samples for the statistical comparison. Conclusion: The method for midazolam using DBS was validated and showed an excellent performance. Excellent correlations were observed when the same collection procedures were used. |
Databáze: | OpenAIRE |
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