Growth and immunolocalisation of the brown alga Ectocarpus in a microfluidic environment
| Autor: | Samuel Boscq, Nino F. Läubli, Bradley J. Nelson, Bénédicte Charrier |
|---|---|
| Přispěvatelé: | Chimie Et Interdisciplinarité : Synthèse, Analyse, Modélisation (CEISAM), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC) |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
chemistry.chemical_classification 0303 health sciences biology Chemistry Cellular differentiation [SDV.BDD.MOR]Life Sciences [q-bio]/Development Biology/Morphogenesis Sporophyte [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] Ectocarpus Polysaccharide biology.organism_classification 01 natural sciences Spore law.invention Cell wall 03 medical and health sciences Confocal microscopy law Fluorescence microscope Biophysics 030304 developmental biology 010606 plant biology & botany |
| DOI: | 10.1101/2021.07.20.453111 |
| Popis: | PDMS chips have proven to be suitable environments for the growth of several filamentous organisms. However, depending on the specimen, the pattern of growth and cell differentiation has been rarely investigated. We monitored the developmental pattern of the brown alga Ectocarpus inside a PDMS lab-on-chip. Two main methods of inoculation of the lab-on-chip were tested, i.e. by injection of spores or by insertion of sporophyte filaments into the chamber. Growth rate, growth trajectory, cell differentiation, and branching were the main development steps that were monitored for 20 days inside 25 μm or 40 μm parallel channels under standard light and temperature conditions. They were shown to be similar to those observed in non-constrained in-vitro conditions. Labelling of Ectocarpus cell wall polysaccharides – both with calcofluor for cellulose, and by immunolocalisation for alginates with monoclonal antibodies–showed expected patterns when compared to open space growth using either epifluorescence or confocal microscopy. Overall this article describes the experimental conditions for observing and studying the basic unaltered processes of brown algal growth using microfluidic technology, which provides the basis for future biochemical and biological research. |
| Databáze: | OpenAIRE |
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