Fluorescence fluctuation-based super-resolution microscopy using multimodal waveguided illumination
| Autor: | Tore Seternes, Daniel Henry Hansen, Firehun Tsige Dullo, Krishna Agarwal, Anish Priyadarshi, Roy A. Dalmo, Ida Sundvor Opstad, Balpreet Singh Ahluwalia, Jean-Claude Tinguely, Sebastian Acuña, Florian Ströhl |
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| Rok vydání: | 2021 |
| Předmět: |
Image quality
Animal Scales 02 engineering and technology 01 natural sciences Fluorescence 010309 optics Optics Salmon 0103 physical sciences Microscopy Fluorescence microscope Animals Lighting Common emitter Physics Total internal reflection Photons Total internal reflection fluorescence microscope Super-resolution microscopy business.industry Optical Imaging 021001 nanoscience & nanotechnology Atomic and Molecular Physics and Optics VDP::Mathematics and natural science: 400::Physics: 430::Electromagnetism acoustics optics: 434 Microscopy Fluorescence VDP::Matematikk og Naturvitenskap: 400::Fysikk: 430::Elektromagnetisme akustikk optikk: 434 Epidermis 0210 nano-technology business Refractive index |
| Zdroj: | Optics express. 29(15) |
| ISSN: | 1094-4087 |
| Popis: | Photonic chip-based total internal reflection fluorescence microscopy (c-TIRFM) is an emerging technology enabling a large TIRF excitation area decoupled from the detection objective. Additionally, due to the inherent multimodal nature of wide waveguides, it is a convenient platform for introducing temporal fluctuations in the illumination pattern. The fluorescence fluctuation-based nanoscopy technique multiple signal classification algorithm (MUSICAL) does not assume stochastic independence of the emitter emission and can therefore exploit fluctuations arising from other sources, as such multimodal illumination patterns. In this work, we demonstrate and verify the utilization of fluctuations in the illumination for super-resolution imaging using MUSICAL on actin in salmon keratocytes. The resolution improvement was measured to be 2.2–3.6-fold compared to the corresponding conventional images. |
| Databáze: | OpenAIRE |
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