Dark field microscopy of the subgingival microflora in insulin-dependent diabetics
| Autor: | B. Seppala, Jukka Ainamo |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Adult
Blood Glucose Pathology medicine.medical_specialty Gingival and periodontal pocket Alveolar Bone Loss Colony Count Microbial Gingiva Gingival Pocket Gastroenterology 03 medical and health sciences 0302 clinical medicine Diabetes mellitus Internal medicine Periodontal Attachment Loss medicine Humans Periodontal Pocket 030212 general & internal medicine Aged Periodontitis Glycated Hemoglobin Microscopy Bacteria business.industry Dental Plaque Index 030206 dentistry Middle Aged medicine.disease Dark field microscopy 3. Good health Diabetes Mellitus Type 1 Clinical attachment loss Spirochaetales Periodontics Hemoglobin Periodontal Index Insulin dependent business Gingival Hemorrhage |
| Zdroj: | Journal of clinical periodontology. 23(2) |
| ISSN: | 0303-6979 |
| Popis: | The subgingival microflora was assessed by means of dark field microscopy in 106 pockets of 47 subjects with long-term insulin-dependent diabetes mellitus (IDD). The microbiota of 55 healthy sulci (probing depth4 mm) and 51 periodontally diseased (probing depthor = 4 and 6 mm) pockets were analyzed. The mean duration of the IDD of the diabetic subjects was 23.7 years (range from 10 to 41 years). The diabetic patients, aged 30-65 years, were under medical treatment at the III Department of Medicine, University of Helsinki Central Hospital, and at 2 clinics of the Helsinki Health Centre. Based upon their long-term medical records, 26 subjects were assessed to have poorly controlled insulin-dependent diabetes mellitus (PIDD) and 21 had controlled insulin-dependent diabetes (CIDD). The PIDD subjects exhibited higher mean blood glucose levels (12.9 +/- 4.6 mmol/l, mean +/- S.D.) than the CIDD subjects (7.9 +/- 3.6 mmol/l) (p0.001, t-test). The mean glycosylated hemoglobin HBA1 (HBA1c) levels were 11.2 +/- 4.6% (10.3 +/- 1.2%) and 8.8 +/- 1.8% (7.7 +/- 1.4%) for PIDD and CIDD subjects, respectively. These differences were statistically significant (p0.01 and p0.001, t-test). For each individual, site-specific recordings were made for plaque index and bleeding index scores, probing depth, loss of attachment and radiographic loss of alveolar bone. Dark field microscopy analysis of the presence of spirochetes, motile rods, cocci, non-motile rods, filaments and fusiforms was performed in the total of 106 pockets. According to the results of the dark field microscopy, the % of spirochetes and motile rods in the periodontally diseased pockets was significantly higher in the PIDD than in the CIDD subjects (9.2 +/- 13.4% and 10.8 +/- 14.3% versus 4.0 +/- 5.2% and 3.1 +/- 3.2%, p0.01 and p0.001, chi 2-test, respectively). Moreover, the PIDD subjects had lower mean %s of coccoid cells in periodontally diseased sites than the CIDD subjects (52.1 +/- 20.8% versus 60.7 +/- 9.0%, p0.001, chi 2-test). |
| Databáze: | OpenAIRE |
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