Modulation of Medium-Chain Fatty Acid Synthesis in Synechococcus sp. PCC 7002 by Replacing FabH with a Chaetoceros Ketoacyl-ACP Synthase
| Autor: | Huiya Gu, Matthew C. Posewitz, Robert E. Jinkerson, Philip E. Schneider, Lyle A. Sisson, Fiona K. Davies |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Chlamydomonas reinhardtii Plant Science lcsh:Plant culture medicine.disease_cause 01 natural sciences 03 medical and health sciences chemistry.chemical_compound medicine lcsh:SB1-1110 Phaeodactylum tricornutum Escherichia coli Fatty acid synthesis Original Research chemistry.chemical_classification type III ketoacyl-ACP synthase biology ATP synthase Synechococcus sp. PCC7002 Chaetoceros Synechococcus sp. PCC 7002 RNAseq biology.organism_classification Synechococcus 030104 developmental biology Enzyme chemistry Biochemistry Medium chain fatty acids biology.protein lauric acid production 010606 plant biology & botany |
| Zdroj: | Frontiers in Plant Science, Vol 7 (2016) Frontiers in Plant Science |
| ISSN: | 1664-462X |
| Popis: | The isolation or engineering of algal cells synthesizing high levels of medium-chain fatty acids (MCFAs) is attractive to mitigate the high clouding point of longer chain fatty acids in algal based biodiesel. To develop a more informed understanding of MCFA synthesis in photosynthetic microorganisms, we isolated several algae from Great Salt Lake and screened this collection for MCFA accumulation to identify strains naturally accumulating high levels of MCFA. A diatom, Chaetoceros sp. GSL56, accumulated particularly high levels of C14 (up to 40%), with the majority of C14 fatty acids allocated in triacylglycerols. Using whole cell transcriptome sequencing and de novo assembly, putative genes encoding fatty acid synthesis enzymes were identified. Enzymes from this Chaetoceros sp. were expressed in the cyanobacterium Synechococcus sp. PCC 7002 to validate gene function and to determine whether eukaryotic enzymes putatively lacking bacterial evolutionary control mechanisms could be used to improve MCFA production in this promising production strain. Replacement of the Synechococcus 7002 native FabH with a Chaetoceros ketoacyl-ACP synthase III increased MCFA synthesis up to fivefold. The level of increase is dependent on promoter strength and culturing conditions. |
| Databáze: | OpenAIRE |
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