The microbiome of the lung and its extracellular vesicles in nonsmokers, healthy smokers and COPD patients
| Autor: | Sunmi Yun, Jun-Pyo Choi, Hyun-Jung Kim, Sei Won Lee, Lokesh Sharma, Kang-Hyun Kim, Jae Seung Lee, Yoon Keun Kim, Yeon-Mok Oh, You-Sun Kim, Charles S. Dela Cruz, Sang Do Lee |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Lung microbiome Clinical Biochemistry Disease Biology Biochemistry Pathogenesis 03 medical and health sciences Extracellular Vesicles Pulmonary Disease Chronic Obstructive 0302 clinical medicine medicine Humans Microbiome Molecular Biology Lung Aged COPD Microbiota Smoking Case-control study Cancer Middle Aged medicine.disease respiratory tract diseases 030104 developmental biology medicine.anatomical_structure 030228 respiratory system Case-Control Studies Immunology Molecular Medicine Original Article Female |
| Zdroj: | Experimental & Molecular Medicine |
| ISSN: | 2092-6413 1226-3613 |
| Popis: | Chronic obstructive pulmonary disease (COPD) is a chronic inflammatory disease, and bacterial infection plays a role in its pathogenesis. Bacteria secrete nanometer-sized extracellular vesicles (EVs), which may induce more immune dysfunction and inflammation than the bacteria themselves. We hypothesized that the microbiome of lung EVs might have distinct characteristics depending on the presence of COPD and smoking status. We analyzed and compared the microbiomes of 13 nonsmokers with normal spirometry, 13 smokers with normal spirometry (healthy smokers) and 13 patients with COPD by using 16S ribosomal RNA gene sequencing of surgical lung tissue and lung EVs. Subjects were matched for age and sex in all groups and for smoking levels in the COPD and healthy smoker groups. Each group included 12 men and 1 woman with the same mean age of 65.5 years. In all groups, EVs consistently showed more operational taxonomic units (OTUs) than lung tissue. In the healthy smoker and COPD groups, EVs had a higher Shannon index and a lower Simpson index than lung tissue and this trend was more prominent in the COPD group. Principal component analysis (PCA) showed clusters based on sample type rather than participants' clinical characteristics. Stenotrophomonas, Propionibacterium and Alicyclobacillus were the most commonly found genera. Firmicutes were highly present in the EVs of the COPD group compared with other samples or groups. Our analysis of the lung microbiome revealed that the bacterial communities present in the EVs and in the COPD group possessed distinct characteristics with differences in the OTUs, diversity indexes and PCA clustering. |
| Databáze: | OpenAIRE |
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