Molecular Typing of Mycobacterium intracellulare Using Pulsed-Field Gel Electrophoresis, Variable-Number Tandem-Repeat Analysis, Mycobacteria Interspersed Repetitive-Unit-Variable-Number Tandem Repeat Typing, and Multilocus Sequence Typing: Molecular Characterization and Comparison of Each Typing Methods
| Autor: | Mi-Sun Park, Taesun Shim, Seong-Han Kim, Bum Joon Kim, Semi Jeon, Nara Lim, Seung-Jik Kwon |
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| Rok vydání: | 2014 |
| Předmět: |
Genetics
biology Public Health Environmental and Occupational Health Strain typing multilocus sequence typing bacterial infections and mycoses biology.organism_classification mycobacteria interspersed repetitive-unit-variable-number tandem-repeat analysis Mycobacterium intracellulare Molecular typing Variable number tandem repeat Infectious Diseases pulsed-field gel electrophoresis variable-number tandem-repeat analysis Pulsed-field gel electrophoresis bacteria Multilocus sequence typing Original Article Typing methods Typing Mycobacterium |
| Zdroj: | Osong Public Health and Research Perspectives |
| ISSN: | 2210-9099 |
| DOI: | 10.1016/j.phrp.2014.04.003 |
| Popis: | ObjectivesMycobacterium intracellulare is the major causative agent of nontuberculous mycobacteria-related pulmonary infections. The strain typing of M. intracellulare is important for the treatment and control of its infections. We compared the discrimination capacity and effective value of four different molecular typing methods.MethodsAntibiotic susceptibility testing, hsp65 and rpoB sequencing, pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), mycobacteria interspersed repetitive-unit-variable-number tandem-repeat analysis (MIRU-VNTR), and VNTR assay targeting 44 M. intracellulare isolates obtained from patients with pulmonary infections were performed.ResultsAll the antibiotic susceptibility patterns had no association with the molecular and sequence types tested in this study; however, the molecular and sequence types were related with each other. PFGE gave best results for discriminatory capacity, followed by VNTR, MLST, and MIRU-VNTR.ConclusionThe high discriminatory power of PFGE, VNTR, and MLST is enough for differentiating between reinfection and relapse, as well as for other molecular epidemiological usages. The MLST could be regarded as a representative classification method, because it showed the clearest relation with the sequence types. |
| Databáze: | OpenAIRE |
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