Pharmacological Potential of Phylogenetically Diverse Actinobacteria Isolated from Deep-Sea Coral Ecosystems of the Submarine Avilés Canyon in the Cantabrian Sea
| Autor: | Fernando Vázquez, Axayacatl Molina, Jonathan Fernández, Luis A. García, Gloria Blanco, J. Palacios, Luis Otero, Verónica González, Alfredo F. Braña, José Luis Acuña, Andreas Kulik, Aida Sarmiento-Vizcaíno |
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| Rok vydání: | 2016 |
| Předmět: |
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Bacterial 0301 basic medicine Cell Survival 030106 microbiology Secondary Metabolism Soil Science Antineoplastic Agents Marine Biology Streptomyces Gas Chromatography-Mass Spectrometry Actinobacteria 03 medical and health sciences Microbial ecology Cell Line Tumor RNA Ribosomal 16S Pseudonocardia Botany Animals Seawater Chromatography High Pressure Liquid Ecosystem Phylogeny Ecology Evolution Behavior and Systematics Invertebrate Bioprospecting Sipuncula Biological Products geography geography.geographical_feature_category Bacteria Base Sequence Ecology biology Coral Reefs Plant Extracts Phylum Biodiversity Coral reef Anthozoa Classification biology.organism_classification Invertebrates Anti-Bacterial Agents Genes Bacterial Spain bacteria |
| Zdroj: | Microbial Ecology. 73:338-352 |
| ISSN: | 1432-184X 0095-3628 |
| DOI: | 10.1007/s00248-016-0845-2 |
| Popis: | Marine Actinobacteria are emerging as an unexplored source for natural product discovery. Eighty-seven deep-sea coral reef invertebrates were collected during an oceanographic expedition at the submarine Avilés Canyon (Asturias, Spain) in a range of 1500 to 4700 m depth. From these, 18 cultivable bioactive Actinobacteria were isolated, mainly from corals, phylum Cnidaria, and some specimens of phyla Echinodermata, Porifera, Annelida, Arthropoda, Mollusca and Sipuncula. As determined by 16S rRNA sequencing and phylogenetic analyses, all isolates belong to the phylum Actinobacteria, mainly to the Streptomyces genus and also to Micromonospora, Pseudonocardia and Myceligenerans. Production of bioactive compounds of pharmacological interest was investigated by high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) techniques and subsequent database comparison. Results reveal that deep-sea isolated Actinobacteria display a wide repertoire of secondary metabolite production with a high chemical diversity. Most identified products (both diffusible and volatiles) are known by their contrasted antibiotic or antitumor activities. Bioassays with ethyl acetate extracts from isolates displayed strong antibiotic activities against a panel of important resistant clinical pathogens, including Gram-positive and Gram-negative bacteria, as well as fungi, all of them isolated at two main hospitals (HUCA and Cabueñes) from the same geographical region. The identity of the active extracts components of these producing Actinobacteria is currently being investigated, given its potential for the discovery of pharmaceuticals and other products of biotechnological interest. |
| Databáze: | OpenAIRE |
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