Indirect immunofluorescence test for serodiagnosis of Legionnaires disease: evidence for serogroup diversity of Legionnaires disease bacterial antigens and for multiple specificity of human antibodies
| Autor: | H W Wilkinson, D D Cruce, B J Fikes |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
Microbiology (medical)
Serotype Fluorescent Antibody Technique Biology Immunofluorescence Serology Microbiology Diagnosis Differential Epitopes Blood serum Antigen Antibody Specificity medicine Humans Serotyping Direct fluorescent antibody Antigens Bacterial Bacteria medicine.diagnostic_test medicine.disease Antibodies Bacterial Virology Legionnaires' disease Bacterial antigen Legionnaires' Disease Research Article |
| Zdroj: | Journal of Clinical Microbiology. 9:379-383 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | Evidence obtained by others who used direct immunofluorescence staining to demonstrate serological differences among strains of Legionnaires disease bacterium prompted this study of parameters influencing the ability of the indirect immunofluorescence test to detect human antibodies to Legionnaires disease bacterium. A total of 25 Legionnaires disease bacterium strains, representing four serogroups, were used as immunofluorescence antigens to test selected human sera. The use of diethyl ether in preparing the antigens was discontinued when it was found that titers against ether-killed group 2 (Togus 1-like) antigens were impossible to determine. Instead, heat-killed suspensions of Legionnaires disease bacterium in 0.5% buffered normal chicken yolk sac were used to show the serogroup diversity of the strains and the serogroup specificity of the antibody response of some, but not all, patients with serological evidence of Legionnaires disease. These studies suggest that multiple antigens should be used in serological tests for Legionnaires disease. Furthermore, the fact that some sera contain antibodies that bind equally well to strains of all four serogroups implies that demonstration of a fourfold increase in titer of paired sera when tested with a single antigen should not be interpreted as evidence of infection with a strain of the same serogroup. |
| Databáze: | OpenAIRE |
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