Upregulation of aryl hydrocarbon receptor nuclear translocator 2 in the hippocampi of post‑stroke depression rats
| Autor: | Chen‑Di Pei, Lu‑Lu Zhang, Shi‑Na Gu, Xu‑Sheng Wang, Wen‑Sheng Wang, Wen‑Qiang Li, Peng‑Ge Fei, Zhao‑Hui Zhang, Jun‑Lin Mu, Jing‑Gui Song, Xiang Yang Zhang |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Hypoxia-Inducible Factor 1 medicine.medical_specialty Aryl hydrocarbon receptor nuclear translocator hypoxia-inducible factor 1 General Biochemistry Genetics and Molecular Biology Open field 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Internal medicine medicine Post-stroke depression General Pharmacology Toxicology and Pharmaceutics cognitive impairment post-stroke depression Messenger RNA Chemistry General Neuroscience Articles General Medicine aryl hydrocarbon receptor nuclear translocator protein 2 hippocampal regeneration 030104 developmental biology Endocrinology Aryl Hydrocarbon Receptor Nuclear Translocator 2 Apoptosis 030220 oncology & carcinogenesis |
| Zdroj: | Biomedical Reports |
| ISSN: | 2049-9442 2049-9434 |
| DOI: | 10.3892/br.2019.1225 |
| Popis: | Aryl hydrocarbon receptor nuclear translocator protein 2 (ARNT2), a member of the basic helix-loop-helix superfamily of transcription factors, may serve a vital role in neuronal survival and cell proliferation via formation of heterodimers with hypoxia-inducible factor-1α. Previous studies indicated that ARNT2 levels were elevated in the brains of ischemic rats; however, the involvement of ARNT2 in post-stroke depression (PSD) rats is not well understood. Therefore, the present study aimed to investigate the levels of ARNT2 in the hippocampi of PSD rats, and to clarify the potential association between ARNT2 and behavioral performance. A PSD rat model was established by middle cerebral artery occlusion (MCAO) followed by a 4-week chronic unpredictable mild stress (CUMS) regimen. A sucrose preference test and open field test (OFT) were conducted, and body weight was measured. In addition, reverse transcription-polymerase chain reaction and immunohistochemistry were performed to measure ARNT expression. Results indicated that MCAO+CUMS rats had lower weight gain, consumed less sucrose and moved less compared with controls. Furthermore, the mRNA and protein levels of ARNT in MCAO+CUMS rats were increased compared with in controls. The sucrose preference index and horizontal movement distance in the OFT were positively correlated with ARNT mRNA level. Thus, from these findings it was suggested that ARNT2 may be positively associated with improvement of cognitive impairment, and therefore may be a potential target in PSD treatment. |
| Databáze: | OpenAIRE |
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