Trypanosomes have divergent kinesin-2 proteins that function differentially in flagellum biosynthesis and cell viability
| Autor: | W. Zacheus Cande, Haiming Wu, Brett M. Haltiwanger, Joel Mancuso, Robert L. Jeng, Matthew D. Welch, Robert L. Douglas, Anna Albisetti |
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| Rok vydání: | 2013 |
| Předmět: |
Cell Survival
1.1 Normal biological development and functioning Trypanosoma brucei brucei Protozoan Proteins Kinesins Trypanosoma brucei Flagellum Medical and Health Sciences Motor protein Trypanosome brucei Underpinning research Intraflagellar transport 2.2 Factors relating to the physical environment Basal body Aetiology biology Kinesin Cell Biology Biological Sciences biology.organism_classification Cell biology Vector-Borne Diseases Infectious Diseases Good Health and Well Being Flagella sense organs Infection Cytokinesis Biogenesis Developmental Biology |
| Zdroj: | Journal of cell science, vol 133, iss 13 |
| ISSN: | 1477-9137 |
| Popis: | Trypanosoma brucei, the causative agent of African sleeping sickness, has a flagellum that is crucial for motility, pathogenicity, and viability. In most eukaryotes, the intraflagellar transport (IFT) machinery drives flagellum biogenesis, and anterograde IFT requires kinesin-2 motor proteins. In this study, we investigated the function of the two T. brucei kinesin-2 proteins, TbKin2a and TbKin2b, in bloodstream form trypanosomes. We found that compared to other kinesin-2 proteins, TbKin2a and TbKin2b show greater variation in neck, stalk, and tail domain sequences. Both kinesins contributed additively to flagellar lengthening. Silencing TbKin2a inhibited cell proliferation, cytokinesis and motility, whereas silencing TbKin2b did not. TbKin2a was localized on the flagellum and colocalized with IFT components near the basal body, consistent with it performing a role in IFT. TbKin2a was also detected on the flagellar attachment zone, a specialized structure that connects the flagellum to the cell body. Our results indicate that kinesin-2 proteins in trypanosomes play conserved roles in flagellar biosynthesis and exhibit a specialized localization, emphasizing the evolutionary flexibility of motor protein function in an organism with a large complement of kinesins. |
| Databáze: | OpenAIRE |
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