Chick chorioallantoic membrane assay as an in vivo model to study the effect of nanoparticle-based anticancer drugs in ovarian cancer
| Autor: | Jonas G. Croissant, Altagracia Conteras, Laurence Raehm, Tammy Yik, Kotaro Matsumoto, Fuyuhiko Tamanoi, Niveen M. Khashab, Laura E. Ratliff, Tan Le Hoang Doan, Carlotta A. Glackin, Yevhen Fatieiev, Shirleen I. Simargi, Chiara Mauriello Jimenez, Sophia Allaf Shahin, Binh Thanh Vu, Jean-Olivier Durand |
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| Přispěvatelé: | Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier ( ICGM ICMMM ), Université Montpellier 1 ( UM1 ) -Université Montpellier 2 - Sciences et Techniques ( UM2 ) -Ecole Nationale Supérieure de Chimie de Montpellier ( ENSCM ) -Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ), Microbiology, Immunology and Molecular Genetics ( UCLA ), University of California at Los Angeles [Los Angeles] ( UCLA ), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Microbiology, Immunology and Molecular Genetics (UCLA), University of California [Los Angeles] (UCLA), University of California-University of California |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
02 engineering and technology Chick Embryo Chorioallantoic Membrane Models Nanotechnology ComputingMilieux_MISCELLANEOUS Cancer Ovarian Neoplasms Drug Carriers Multidisciplinary Tumor [CHIM.ORGA]Chemical Sciences/Organic chemistry Chemistry 021001 nanoscience & nanotechnology Ovarian Cancer 3. Good health Chorioallantoic membrane 5.1 Pharmaceuticals Drug delivery embryonic structures Medicine Biological Assay Female Development of treatments and therapeutic interventions 0210 nano-technology Biotechnology medicine.drug Stromal cell Science Bioengineering Models Biological Article Cell Line 03 medical and health sciences Targeted therapies Rare Diseases In vivo [ CHIM.ORGA ] Chemical Sciences/Organic chemistry Cell Line Tumor medicine Animals Humans Doxorubicin medicine.disease Biological Orphan Drug 030104 developmental biology Cell culture Cancer research Nanoparticles Ovarian cancer |
| Zdroj: | Scientific reports, vol 8, iss 1 Scientific Reports Scientific Reports, Nature Publishing Group, 2018, 8 (1), pp.8524. 〈10.1038/s41598-018-25573-8〉 Scientific Reports, Vol 8, Iss 1, Pp 1-10 (2018) Scientific Reports, Nature Publishing Group, 2018, 8 (1), pp.8524. ⟨10.1038/s41598-018-25573-8⟩ |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-018-25573-8〉 |
| Popis: | New therapy development is critically needed for ovarian cancer. We used the chicken egg CAM assay to evaluate efficacy of anticancer drug delivery using recently developed biodegradable PMO (periodic mesoporous organosilica) nanoparticles. Human ovarian cancer cells were transplanted onto the CAM membrane of fertilized eggs, resulting in rapid tumor formation. The tumor closely resembles cancer patient tumor and contains extracellular matrix as well as stromal cells and extensive vasculature. PMO nanoparticles loaded with doxorubicin were injected intravenously into the chicken egg resulting in elimination of the tumor. No significant damage to various organs in the chicken embryo occurred. In contrast, injection of free doxorubicin caused widespread organ damage, even when less amount was administered. The lack of toxic effect of nanoparticle loaded doxorubicin was associated with specific delivery of doxorubicin to the tumor. Furthermore, we observed excellent tumor accumulation of the nanoparticles. Lastly, a tumor could be established in the egg using tumor samples from ovarian cancer patients and that our nanoparticles were effective in eliminating the tumor. These results point to the remarkable efficacy of our nanoparticle based drug delivery system and suggests the value of the chicken egg tumor model for testing novel therapies for ovarian cancer. がんの個別化医療を可能にする患者癌由来の鶏卵モデル. 京都大学プレスリリース. 2018-06-06. |
| Databáze: | OpenAIRE |
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