Levels of Gamma Interferon and Interleukin-4 Are Inversely Related to the Levels of Their Corresponding Autoantibodies in Patients with Lower Respiratory Tract Infection
| Autor: | Lars Lindquist, Rihab A. Elkarim, Moiz Bakhiet, Hans Link, Carl Granert |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Adult
Male medicine.medical_treatment Immunology Biology Microbiology Peripheral blood mononuclear cell Proinflammatory cytokine Interferon-gamma medicine Humans Interferon gamma Respiratory Tract Infections Interleukin 4 Aged Autoantibodies Aged 80 and over Host Response and Inflammation Respiratory tract infections Autoantibody Middle Aged Infectious Diseases Cytokine biology.protein Female Parasitology Interleukin-4 Antibody medicine.drug |
| Zdroj: | Infection and Immunity. 67:3051-3054 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.67.6.3051-3054.1999 |
| Popis: | To study the involvement of cytokines and their corresponding autoantibodies (Aabs) in inflammatory mechanisms in patients with lower respiratory tract infections, blood samples were taken from patients at the time of admission to the hospital and before treatment. Cell-released capturing enzyme-linked immunosorbent assay was used to measure the levels of gamma interferon (IFN-γ) and interleukin-4 (IL-4) produced spontaneously by peripheral mononuclear cells (PMNC). ELISA was used to measure Aabs to these cytokines in sera. The levels of both cytokines were inversely related to the levels of their corresponding Aabs. While a high level of IFN-γ was observed together with a low level of anti-IFN-γ Aab, decreased IL-4 levels were observed with increased levels of Aabs to IL-4. Immunoglobulins were purified, digested to obtain Fab fragments, and tested for specificity and cross-reactivity. The Aabs and their Fab fragments were tested in cytokine biological assays and showed neutralizing effects. Our data demonstrated increased levels of the proinflammatory cytokine IFN-γ and decreased release of the anti-inflammatory cytokine IL-4 during early presentation of lower respiratory tract infection. The levels of these cytokines were inversely related to the levels of their corresponding Aabs that exhibited regulatory effects on the cytokine biological function in vitro. |
| Databáze: | OpenAIRE |
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