Cytochalasin-B-Inducible Nanovesicle Mimics of Natural Extracellular Vesicles That Are Capable of Nucleic Acid Transfer
| Autor: | Marina A. Zenkova, Alexandra Neumestova, Vera Matveeva, Anastasiya L. Oshchepkova, Valentin V. Vlassov, K. N. Morozova, Elena Kiseleva, Lyudmila Artemyeva |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
nanovesicles
lcsh:Mechanical engineering and machinery Sonication Cell mesenchymal stem cells (MSCs) nucleic acid delivery Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine lcsh:TJ1-1570 freezing and thawing Electrical and Electronic Engineering Cytochalasin B 030304 developmental biology 0303 health sciences Nuclease biology Oligonucleotide Mechanical Engineering Vesicle Extracellular vesicle extracellular vesicles (EVs) cytochalasin B medicine.anatomical_structure chemistry Control and Systems Engineering 030220 oncology & carcinogenesis biology.protein Nucleic acid Biophysics |
| Zdroj: | Micromachines Volume 10 Issue 11 Micromachines, Vol 10, Iss 11, p 750 (2019) |
| ISSN: | 2072-666X |
| DOI: | 10.3390/mi10110750 |
| Popis: | Extracellular vesicles provide cell-to-cell communication and have great potential for use as therapeutic carriers. This study was aimed at the development of an extracellular vesicle-based system for nucleic acid delivery. Three types of nanovesicles were assayed as oligonucleotide carriers: mesenchymal stem cell-derived extracellular vesicles and mimics prepared either by cell treatment with cytochalasin B or by vesicle generation from plasma membrane. Nanovesicles were loaded with a DNA oligonucleotide by freezing/thawing, sonication, or permeabilization with saponin. Oligonucleotide delivery was assayed using HEK293 cells. Extracellular vesicles and mimics were characterized by a similar oligonucleotide loading level but different efficiency of oligonucleotide delivery. Cytochalasin-B-inducible nanovesicles exhibited the highest level of oligonucleotide accumulation in HEK293 cells and a loading capacity of 0.44 ± 0.05 pmol/µ g. The loaded oligonucleotide was mostly protected from nuclease action. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|