WNK1 and p38-MAPK distribution in ionocytes and accessory cells of euryhaline teleost fish implies ionoregulatory function
| Autor: | M. Spieker, William S. Marshall, Regina R. F. Cozzi |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
QH301-705.5 Science Fundulus heteroclitus Biology General Biochemistry Genetics and Molecular Biology Chloride cell NKCC 03 medical and health sciences Accessory cell Freshwater Seawater Biology (General) CFTR WNK1 Protein kinase A Epithelial polarity Euryhaline Apical membrane Cystic fibrosis transmembrane conductance regulator Cell biology Cytosol 030104 developmental biology Biochemistry Hypersaline p38MAPK biology.protein General Agricultural and Biological Sciences Cotransporter Teleost fish Forskolin Immunocytochemistry Research Article |
| Zdroj: | Biology Open Biology Open, Vol 6, Iss 7, Pp 956-966 (2017) |
| ISSN: | 2046-6390 |
| DOI: | 10.1242/bio.024232 |
| Popis: | Ionocytes of euryhaline teleost fish secrete NaCl, under regulation by serine and threonine kinases, including with-no-lysine kinase (WNK1) and p38 mitogen-activated protein kinase (MAPK). Mummichogs (Fundulus heteroclitus L.) were acclimated to freshwater (FW), full strength seawater (SW) and hypersaline conditions (2SW). Immunocytochemistry of ionocytes in opercular epithelia of fish acclimated to SW and 2SW revealed that WNK1-anti-pT58 phosphoantibody localized strongly to accessory cells and was present in the cytosol of ionocytes, close to cystic fibrosis transmembrane conductance regulator (CFTR) in the apical membrane and the sodium potassium 2 chloride cotransporter (NKCC) in the basolateral membrane. In FW acclimated fish, WNK1 localized to a sub-apical zone, did not colocalize with apical membrane-located sodium chloride cotransporter (NCC), and typically was present in one cell of paired ionocytes and in some single ionocytes. Forskolin treatment (10 μM, 30 min) increased WNK1 immunofluorescence in SW ionocytes only, while hypertonicity had little effect, compared to controls. Anti-p38-MAPK antibody localized to the cytosolic compartment. The distribution of WNK1 and p38MAPK is consistent with a proximal position in regulatory cascades, rather than directly affecting transporters. The strong staining of accessory cells by WNK1 phosphoantibody infers an osmoregulatory function for WNK. Summary: Fish opercular epithelium ionocytes and accessory cells have WNK family kinases that may regulate paracellular and transcellular ion transport. |
| Databáze: | OpenAIRE |
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