Activity of the Antimicrobial Peptide and Thanatin Analog S-thanatin on Clinical Isolates of Klebsiella pneumoniae Resistant to Conventional Antibiotics with Different Structures
| Autor: | Zilong Shen, Guoqiu Wu, Jiaxuan Ding, Linxian Li, Rui Zhao, Hai-liang Wang |
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| Rok vydání: | 2009 |
| Předmět: |
medicine.drug_class
Tetracycline Klebsiella pneumoniae Cefepime Antibiotics Microbial Sensitivity Tests Drug resistance Biology Peptides Cyclic Applied Microbiology and Biotechnology Microbiology Heteroptera Drug Resistance Bacterial medicine Animals Microbial Viability Drug Synergism General Medicine Antimicrobial biology.organism_classification Anti-Bacterial Agents Klebsiella Infections Ciprofloxacin Gentamicin Antimicrobial Cationic Peptides medicine.drug |
| Zdroj: | Current Microbiology. 59:147-153 |
| ISSN: | 1432-0991 0343-8651 |
| Popis: | The treatment of infections caused by bacteria resistant to the vast majority of antibiotics is a challenge worldwide. To evaluate the effect of S-thanatin (an analog of thanatin, a cationic antimicrobial peptide isolated from the hemipteran insect Podisus maculiventris) against microbial resistant to antibiotics, we studied its bactericidal kinetics, synergistic effect, resistance, and activity on clinical isolates of Klebsiella pneumoniae resistant to conventional antibiotics with different structures. The bactericidal rate of S-thanatin was more than 99% against K. pneumoniae ATCC 700603 when bacterial cultures were monitored for 60 min. The peptide was synergistic with beta-lactam cefepime in most of the clinical MDR isolates tested (7/8). An average value of FIC was 0.3708. No synergy was found between the peptide and amoxicillin, gentamycin, tetracycline, or ciprofloxacin in all bacteria tested. A total of 48 isolates of K. pneumoniae with different resistance spectrum tested was susceptible to S-thanatin. The MICs were 6.25-25 mug/ml. No significant difference in the MICs of S-thanatin between the sensitive isolates and the resistant isolates to single antibiotic was observed (P > 0.05). The resistance of K. pneumoniae ATCC 700603 to S-thanatin was slightly higher, when cultured at sub-inhibitory concentration for 5 days. S-thanatin may be an attractive candidate for developing into an antimicrobial agent. |
| Databáze: | OpenAIRE |
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