Non-invasive in-vivo 3-D imaging of small animals using spatially filtered enhanced truncated-correlation photothermal coherence tomography
| Autor: | Sohrab Roointan, Andreas Mandelis, Pantea Tavakolian |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Materials science Mice Nude lcsh:Medicine Article Correlation Mice 03 medical and health sciences Imaging Three-Dimensional 0302 clinical medicine In vivo Animals Humans Imaging science Optical techniques lcsh:Science Image resolution Multidisciplinary Phantoms Imaging lcsh:R Imaging and sensing Photothermal therapy Applied physics 030104 developmental biology Cancer imaging lcsh:Q Olfactory Lobe Tomography Tomography X-Ray Computed Biomedical engineering Algorithms 030217 neurology & neurosurgery Coherence (physics) |
| Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-10 (2020) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | We present enhanced truncated-correlation phototothermal coherence tomography (eTC-PCT) for non-invasive three-dimensional imaging of small animals. Tumor detection is reported in a mouse thigh by injecting cancerous cells in the thigh followed by eTC-PCT imaging. Detection of the tumor 3 days after injection may lead to potential for using the eTC-PCT method for cancer treatment studies. eTC-PCT was also applied successfully to non-invasive in-vivo mouse brain structural imaging. A unique spatial-gradient-gate adaptive filter was introduced in a scanned mode along the (x,y) coordinates of camera images from different sub-cranial depths, revealing absorber true spatial extent from diffusive photothermal images and restoring pre-diffusion lateral image resolution beyond the Rayleigh criterion limit in diffusion-wave imaging science. The spatial resolution and contrast enhancement demonstrated in photothermal in-vivo and ex-vivo images of the mouse brain revealed not only vascular structures but also other brain structures, such as the brain hemispheres, cerebellum, and olfactory lobes. |
| Databáze: | OpenAIRE |
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