Mind Bomb-Binding Partner RanBP9 Plays a Contributory Role in Retinal Development

Autor: Yun Mi Jeong, Myungchull Rhee, Sateesh Maddirevula, Hyung Goo Kim, Maivannan Thiruvarangan, Cheol-Hee Kim, Mi Sun Lee, Young Ki Bae, Kyeong Won Yoo
Rok vydání: 2017
Předmět:
0301 basic medicine
Neurogenesis
Ubiquitin-Protein Ligases
Ependymoglial Cells
retinogenesis
Notch signaling pathway
Down-Regulation
ELAV-Like Protein 3
Article
Retina
03 medical and health sciences
chemistry.chemical_compound
Two-Hybrid System Techniques
Chlorocebus aethiops
Basic Helix-Loop-Helix Transcription Factors
medicine
Animals
Proliferation Marker
RanBP9
Cyclin-Dependent Kinase Inhibitor p57
Molecular Biology
Zebrafish
Adaptor Proteins
Signal Transducing
Cell Proliferation
Gene knockdown
biology
Brain
Nuclear Proteins
Retinal
Cell Biology
General Medicine
Anatomy
Zebrafish Proteins
medicine.disease
biology.organism_classification
Cell biology
Cytoskeletal Proteins
mind bomb
030104 developmental biology
medicine.anatomical_structure
chemistry
Gene Knockdown Techniques
COS Cells
Retinal dysplasia
Retinal Dysplasia
notch
Zdroj: Molecules and Cells
ISSN: 0219-1032
1016-8478
DOI: 10.14348/molcells.2017.2308
Popis: Ran-binding protein family member, RanBP9 has been reported in various basic cellular mechanisms and neuropathological conditions including schizophrenia. Previous studies have reported that RanBP9 is highly expressed in the mammalian brain and retina; however, the role of RanBP9 in retinal development is largely unknown. Here, we present the novel and regulatory roles of RanBP9 in retinal development of a vertebrate animal model, zebrafish. Zebrafish embryos exhibited abundant expression of ranbp9 in developing brain tissues as well as in the developing retina. Yeast two-hybrid screening demonstrated the interaction of RanBP9 with Mind bomb, a component of Notch signaling involved in both neurogenesis and neural disease autism. The interaction is further substantiated by co-localization studies in cultured cells. Knockdown of ranbp9 resulted in retinal dysplasia with defective proliferation of retinal cells, downregulation of neuronal differentiation marker huC, elevation of neural proliferation marker her4, and alteration of cell cycle marker p57kip2. Expression of the Müller glial cell marker glutamine synthase was also affected in knockdown morphants. Our results suggest that Mind bomb-binding partner RanBP9 plays a role during retinal cell development of zebrafish embryogenesis.
Databáze: OpenAIRE
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