Grass Pollen Immunotherapy Induces Mucosal and Peripheral IL-10 Responses and Blocking IgG Activity
| Autor: | Petra A. Wachholz, Samantha Walker, Stephen J. Till, Mikila R. Jacobson, Steven Q. Staple, Stephen R. Durham, R. C. Aalberse, Louisa K. Wilcock, J.N. Francis, Kayhan T. Nouri-Aria |
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| Přispěvatelé: | Landsteiner Laboratory |
| Rok vydání: | 2004 |
| Předmět: |
Adult
Male Allergy medicine.medical_treatment Immunology medicine.disease_cause Immunoglobulin E Binding Competitive Immunoglobulin G Phleum Allergen Double-Blind Method medicine Humans Immunology and Allergy RNA Messenger Antibodies Blocking Cells Cultured Plant Proteins B-Lymphocytes biology Immune Sera Rhinitis Allergic Seasonal Immunotherapy Allergens medicine.disease biology.organism_classification Interleukin-10 Up-Regulation Nasal Mucosa Interleukin 10 Desensitization Immunologic biology.protein Pollen Female Antibody |
| Zdroj: | Journal of immunology (Baltimore, Md., 172(5), 3252-3259. American Association of Immunologists |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | T regulatory cells and IL-10 have been implicated in the mechanism of immunotherapy in patients with systemic anaphylaxis following bee stings. We studied the role of IL-10 in the induction of clinical, cellular, and humoral tolerance during immunotherapy for local mucosal allergy in subjects with seasonal pollinosis. Local and systemic IL-10 responses and serum Ab concentrations were measured before/after a double-blind trial of grass pollen (Phleum pratense, Phl P) immunotherapy. We observed local increases in IL-10 mRNA-positive cells in the nasal mucosa after 2 years of immunotherapy, but only during the pollen season. IL-10 protein-positive cells were also increased and correlated with IL-10 mRNA+ cells. These changes were not observed in placebo-treated subjects or in healthy controls. Fifteen and 35% of IL-10 mRNA signals were colocalized to CD3+ T cells and CD68+ macrophages, respectively, whereas only 1–2% of total CD3+ cells and 4% of macrophages expressed IL-10. Following immunotherapy, peripheral T cells cultured in the presence of grass pollen extract also produced IL-10. Immunotherapy resulted in blunting of seasonal increases in serum allergen Phl p 5-specific IgE, 60- to 80-fold increases in Phl p 5-specific IgG, and 100-fold increases in Phl p 5-specific IgG4. Post-immunotherapy serum exhibited inhibitory activity, which coeluted with IgG4, and blocked IgE-facilitated binding of allergen-IgE complexes to B cells. Both the increases in IgG and the IgG “blocking” activity correlated with the patients’ overall assessment of improvement. Thus, grass pollen immunotherapy may induce allergen-specific, IL-10-dependent “protective” IgG4 responses. |
| Databáze: | OpenAIRE |
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