An accurate and rapid single step protocol for enumeration of cytokine positive T lymphocytes
| Autor: | Lili Wang, Deepa Rajagopal, Linhua Tian, Luisa Saraiva, Shiqiu Xiong, Sandrine Vessillier, Jonathan Campbell |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cell phenotype Chemistry medicine.medical_treatment 0206 medical engineering Single step 02 engineering and technology 020601 biomedical engineering Article 03 medical and health sciences 030104 developmental biology Cytokine Immune system mental disorders Immunology medicine Enumeration Secretion Intracellular CD8 |
| Zdroj: | J Immunol Regen Med |
| ISSN: | 2468-4988 |
| Popis: | Accurate determination of cellular subsets that secrete particular cytokine(s) is a significant parameter for functional characterization of an immunological response. The present study was conducted to develop a method for simultaneous measurement of intracellular cytokine positive CD4 and CD8 positive T lymphocytes in a single tube, with a no-wash protocol. We report here the development of a simplified, rapid procedure for precise enumeration of cytokine positive T lymphocytes using BD Trucount tubes. This single step protocol for accurate enumeration of cytokine positive T lymphocytes, will allow for better characterization of immune cell phenotype and function. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect