A Novel In Vivo Model to Study Impaired Tissue Regeneration Mediated by Cigarette Smoke
| Autor: | Miguel L. Allende, Myra N. Chávez, Miguel Miranda, Marjorie Alvarez, José T. Egaña, Geraldine Aedo |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male lcsh:Medicine Physiology Embryonic Development Tobacco smoke Article Fin regeneration 03 medical and health sciences In vivo Smoke Medicine Animals Respiratory system lcsh:Science Zebrafish Wound Healing Multidisciplinary biology Dose-Response Relationship Drug business.industry Regeneration (biology) lcsh:R Cancer Tobacco Products biology.organism_classification medicine.disease Disease Models Animal 030104 developmental biology Toxicity lcsh:Q Female business |
| Zdroj: | Scientific Reports Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018) |
| ISSN: | 2045-2322 |
| Popis: | Cigarette smoke is associated with several pathologies including chronic respiratory diseases and cancer. In addition, exposure to cigarette smoke is correlated with impaired wound healing, where a significant decrease in the regenerative capacity of smokers is well documented and broadly considered a negative risk factor after trauma or surgery. So far, some in vitro and in vivo models have been described to study how exposure to cigarette smoke diminishes the regenerative potential in different organisms. However, although useful, many of these models are difficult and expensive to implement and do not allow high-throughput screening approaches. In order to establish a reliable and accessible model, we have evaluated the effects of cigarette smoke extract (CSE) on zebrafish development and regeneration. In this work, zebrafish embryos and larvae were exposed to low doses of aqueous CSE showing severe developmental abnormalities in a dose-dependent manner. Furthermore, when adult zebrafish were subjected to caudal fin amputation, we observed a significant decrease in the regenerative capacity of animals exposed to CSE. The effect was exacerbated in male and aged fish compared to female or young organisms. The establishment of a zebrafish model to assess the consequences of cigarette smoke and its effects on animal physiology could provide a new tool to study the underlying mechanisms involved in impaired tissue regeneration, and aid the development of novel approaches to treat complications associated with cigarette smoke toxicity. |
| Databáze: | OpenAIRE |
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