Deuteration Aiming for Neutron Scattering
| Autor: | Hirokazu Yagi, Koichi Kato, Masahiro Shimizu, Yasuhiro Yunoki, Maho Yagi-Utsumi, Reiko Urade, Ken Morishima, Aya Okuda, Nobuhiro Sato, Rintaro Inoue, Tomohide Saio, Masaaki Sugiyama |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Materials science Hydrogen chemistry.chemical_element Neutron scattering lcsh:Physiology 03 medical and health sciences Kinetic isotope effect Neutron lcsh:QH301-705.5 lcsh:QP1-981 030102 biochemistry & molecular biology Scattering neutron scattering technology industry and agriculture General Medicine lcsh:QC1-999 contrast matching method Method and Protocol 030104 developmental biology lcsh:Biology (General) Deuterium chemistry Chemical physics protein deuteration lcsh:Physics |
| Zdroj: | Biophysics and Physicobiology Biophysics and Physicobiology, Vol 18 (2021) |
| ISSN: | 2189-4779 |
| Popis: | The distinguished feature of neutron as a scattering probe is an isotope effect, especially the large difference in neutron scattering length between hydrogen and deuterium. The difference renders the different visibility between hydrogenated and deuterated proteins. Therefore, the combination of deuterated protein and neutron scattering enables the selective visualization of a target domain in the complex or a target protein in the multi-component system. Despite of this fascinating character, there exist several problems for the general use of this method: difficulty and high cost for protein deuteration, and control and determination of deuteration ratio of the sample. To resolve them, the protocol of protein deuteration techniques is presented in this report. It is strongly expected that this protocol will offer more opportunity for conducting the neutron scattering studies with deuterated proteins. |
| Databáze: | OpenAIRE |
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