Extraction and identification of fungal pigment from Penicillium europium using different spectral studies
| Autor: | Ibrahim Ahmed Shaikh, Ali Mohamed Alshabi, Muazzam Sheriff Maqbul, Tasneem Mohammed, Uday M. Muddapur, R. S. Bennur, Areej Dawoud, Sunil S. More, Aejaz A. Khan, S. M. Shakeel Iqubal, Yahya S. Alqahtani, Awad Mohammed Al-Qahtani |
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| Rok vydání: | 2021 |
| Předmět: |
Pigments
Science (General) food.ingredient chemistry.chemical_element 02 engineering and technology Fungus 010501 environmental sciences Benzoquinone 01 natural sciences Q1-390 Pigment chemistry.chemical_compound food Food science Penicillium europium Biotransformation 0105 earth and related environmental sciences Multidisciplinary biology Food additive Extraction (chemistry) Fungal pigment 021001 nanoscience & nanotechnology biology.organism_classification chemistry visual_art Penicillium visual_art.visual_art_medium Longifolene 0210 nano-technology Europium |
| Zdroj: | Journal of King Saud University: Science, Vol 33, Iss 4, Pp 101437-(2021) |
| ISSN: | 1018-3647 |
| DOI: | 10.1016/j.jksus.2021.101437 |
| Popis: | Objective There is a growing demand for colourants of natural origin in the food, pharmaceutical, cosmetic and textile sectors. Previously, our group has screened a fungal species from forest soil, identified as Penicillium europium. The isolated fungus transformed the longifolene into various metabolites, of which 12 were isolated in pure form, with potential to be utilized in the perfumery industry. This study aimed to isolate and identify novel fungal pigments from Penicillium europium. Methods The current study showcases the extraction and identification of fungal pigment from Penicillium europium using different spectral studies. The strain was isolated from forest soil, Western Ghats, India, and was found to be capable of using longifolene as the sole carbon source. The yellowish pink coloured pigment-producing fungal strain was identified as Penicillium europium. Further, the pinkish pigment was extracted, purified, and using spectral studies like UV, IR, NMR and Mass, the structure of the pure pigment was identified. Results The pure pigment structure was analyzed and tentatively confirmed as 2-(1,5, dimethyl hexyl)-3,5-dimethyl-6-hydro-1,4-benzoquinone having the molecular formula C16H24O3. Toxicity study using LD50 on Albino rats revealed that the pigment had no toxic effect on rats. Conclusion Penicillium europium synthesized pigments could contribute to biotechnology and add value to the food, feed, and pharmaceutical industries. They can be used for various industrial applications, for example, as dyes for textile and non-textile substrates such as paper, leather, coatings and paints, in cosmetics, and food additives. Negative cytotoxicity result inferred that the pigment could be a potential replacement for hazardous synthetic dyes. |
| Databáze: | OpenAIRE |
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